SP5159P EDG-1 / S1PR1 / S1P1 antibody
see related secondary antibodiessee all 13 EDG-1 / S1PR1 / S1P1 products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti EDG-1 / S1PR1 / S1P1
Synonyms
EDG1, D1S3362, CHEDG1, ECGF1, , Sphingosine 1-phosphate receptor 1, Endothelial differentiation G-protein coupled receptor 1
Product review
Please read our product review about EDG-1 / S1PR1 / S1P1 : Antibodies to Endothelial Differentiation Gene Receptors (EDG-1,-2,-4,-5,-6,-7 and -8).
Product Description
Rabbit anti EDG-1 / S1PR1 / S1P1 , Presentation: Aff - Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP5159P |
| Swiss Prot. No. | P21453 |
| Quantity | 0.1 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP5159P.pdf |
Datasheet Extract
| Alternate names | EDG1 |
| Concentration | 1.0 mg/ml |
| Background | Sphingosine 1-phosphate (S1P) is bioactive lipid derived from the metabolism of sphingomyelin. S1P is predominately synthesized, stored, and released from platelets. When released, this molecule stimulates signaling pathways via G-protein coupled receptors. One such receptor is the heterotrimeric guanine nucleotide binding protein shingosine 1-phosphate receptor type 1 (S1P1,also known as EDG1).
Research has demonstrated that S1P regulates numerous cellular responses including, wound healing, cell growth, prevention of apoptosis, vascular maturation and cellular migration and survival. In studies involving S1P1 deficient embryos, blood vessels where incompletely maturated with smooth muscles cells, resulting in embryonic death. In contrast, a separate study demonstrated that overexpression of S1P1 led to the stimulation of cell migration. |
| Immunogen | Synthetic Peptide: S(359) H P Q K D D G D N P E T I(372) corresponding to amino acid residues 359-372 from mouse S1P1. Swiss Num.: P21453 Remarks: This sequence is completely conserved in rat and 92% conserved in human S1P1. This peptide (SP5159CP) is available for use in Neutralization and Control experiments |
| Format | State: Liquid purified IgG fraction. Purification: Epitope affinity chromatography. BufferSystem: PBS containing 1 mg/ml BSA as stabilizer and 0.05% sodium azide as preservative. |
| Applications | Immunofluorescence: 5 µg/ml, demonstrates that S1P1 is localized to the plasma membrane.
Immunoprecipitation. Western Blot: 1 µg/ml, detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. |
| Specificity | SP5159P detects S1P1 protein in transfected human cell samples.
This antibody shows no cross-reactivity to S1P3. |
| Storage | Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Pictures | Figure 1. Imunofluorescence staining of S1P1 in transfected CCL39 cells using SP5159P. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP5159P EDG-1 / S1PR1 / S1P1 '.
