SP5302P FOS / C-FOS antibody
see related secondary antibodiessee all 35 FOS / C-FOS products
0.2 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti FOS / C-FOS
Synonyms
FOS, G0S7, Cellular oncogene fos, G0/G1 switch regulatory protein 7, Proto-oncogene protein c-fos,
Product Description
Rabbit anti FOS / C-FOS , Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Mouse ( Ms ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | SP5302P |
| Swiss Prot. No. | P01101 |
| Quantity | 0.2 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP5302P.pdf |
Datasheet Extract
| Background | Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, (fos-related antigen) Fra1, and Fra2.
Fos and Jun dimerize to form Activator Protein-1 (AP-1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13-acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the 'leucine-zipper' motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP-1 dependent genes. |
| Immunogen | Synthetic Peptide: S(4) G F N A D Y E A S S S R C(17) corresponding to amino acid residues 4-17 from human cFos. Swiss Num.: P01101 Remarks: This sequence is completely conserved in murine cFos. |
| Format | State: Lyophilized purified IgG fraction containing 0.05% sodium azide as preservative Purification: Epitope affinity chromatography. Reconstitution: Restore with PBS. |
| Applications | Western Blot: 10 µg/ml, detects an ~60 kDa protein representing cFos in NIH 3T3 serum stimulated cells. |
| Specificity | SP5302P detects cFos from mouse cells and tissues. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Biochimica et Biophysica Acta, 1072: 129-157, 1991.
2. Critical Reviews in Eukaryotic Gene Expression, 4(1): 55-116, 1994. |
| Pictures | Figure 1. Western blot of cFos on NIH 3T3 cell lysate using SP5302P. Lane 1 shows non-immune serum and lane 2 shows immune serum. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP5302P FOS / C-FOS'.
