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SP5349P K+-dependent, Na Ca Exchanger 2 (NCKX2) antibody
see related secondary antibodiessee all 4 K+-dependent, Na Ca Exchanger 2 (NCKX2) products
0.2 ml / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti K+-dependent, Na Ca Exchanger 2 (NCKX2) -
Product Description
Rabbit anti K+-dependent, Na Ca Exchanger 2 (NCKX2) -, Presentation: Aff - Purified. Product is tested for Frozen sections ( C ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP5349P |
| Quantity | 0.2 ml |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP5349P.pdf |
Datasheet Extract
| Polyclonal Antibody to Rat //K+-dependent, Na Ca Exchanger 2 (NCKX2) | |
| Concentration | 1.0 mg/ml |
| Background | Sodium/calcium-potassium exchanger proteins (NCKX1) have been reported to occur in retinal photoreceptors and in brain (NCKX2). Unlike the abundant and widely expressed sodium/calcium exchanger which catalyzes the exchange of 3 sodium ions for 1 calcium ion to modulate intracellular calcium levels, NCKX isoforms exchange 4 sodium ions for 1 calcium and 1 potassium ion. The sodium/calcium-potassium exchanger uses the transmembrane sodium gradient to catalyze countertransport of calcium in a potassium-dependent mechanism against its electrochemical gradient. It is hypothesized that NCKX2 plays a significant role in calcium homeostasis and the regulation of neuronal function. |
| Immunogen | Synthetic Peptide: D(90) L N D K I R D Y T P Q P(102) corresponding to amino acid residues 90-102 from rat NCKX2. Remarks: This sequence is predicted to be on the extracellular side of the plasma membrane. SP5349P immunizing peptide (SP5349CP) is available for use in Neutralization and Control experiments. |
| Format | State: Liquid purified IgG fraction. Purification: Epitope affinity chromatography. BufferSystem: PBS containing 0.05% sodium azide as preservative and 1 mg/ml BSA as stabilizer. |
| Applications | Western Blot: 1/100, detects a 78 kDa protein representing recombinant rat NCKX2.
Immunohistochemistry on frozen sections: 1/100, staining of rat NCKX2 in HEK cells transfected with the rat gene with this antibody results in staining of the plasma membrane. |
| Specificity | This antibody detects rat sodium/calcium-potassium exchanger (NCKX2) overexpressed in HEK cells transfected with the rat gene. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. J. Biol. Chem., Vol. 277, Issue 50, 48923-48930, December 13, 2002.
2. JBC 277(50):48923-48930, 2002. 3. The Journal of Neuroscience, 23(37)11673-11680, Dec 2003 4. Cell Calcium, 18(4): 322-330, 1995. 5. J. Biol. Chem., 273(7): 4155-4162, 1998. 6. Science, 250: 562-565, 1990. |
| Pictures | Figure 1. Immunlocalization of NCX2 in rat brain using SP5349P antibody. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 170,00 € | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160,00 € | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160,00 € | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / 170,00 € | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170,00 € | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 200,00 € | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'SP5349P K+-dependent, Na Ca Exchanger 2 (NCKX2)'.
