SP5355P S100A1 / Protein S100-A1 antibody
see related secondary antibodiessee all 21 S100A1 / Protein S100-A1 products
0.1 mg / US$ 455
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti S100A1 / Protein S100-A1
Synonyms
S100 Calcium-binding protein A1, S100A, S-100 protein alpha chain, , S-100 protein alpha subunit
Product review
Please read our product review about S100A1 / Protein S100-A1 : Antibodies to S100 Family.
Product Description
Rabbit anti S100A1 / Protein S100-A1 , Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Bovine ( Bov ), Human ( Hu ), Mouse ( Ms ), Por ( Por ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP5355P |
| Swiss Prot. No. | P23297 |
| Quantity | 0.1 mg |
| Price | US$ 455 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP5355P.pdf |
Datasheet Extract
| Background | The S100 protein is a low-molecular-weight, acidic and calcium binding protein that in functional form exist in dimers. S100 has two subunits: S100-alpha (94 aa; human chromosome 1) and S100-beta (92 aa; human chromosome 21) that forms as either homodimers (alpha-alpha known as S-100a(0) or beta-beta known as S-100b) or as heterodimers (known as S-100a) of ~21 kDa. S100-alpha and -beta chains show ~58% sequence identity and are both highly conserved among species. S100-alpha was originally believed to be localized to the CNS, but studies have shown it to be found in numerous tissues including cardiac, skeletal and vascular smooth muscle cells. |
| Immunogen | Purified S100-alpha protein from human pectoral muscle cells Swiss Num.: P23297 |
| Format | State: Lyophilized Ig fraction Purification: Epitope affinity chromatography BufferSystem: PBS containing 30 mg/ml BSA and 0.05% sodium azide |
| Applications | ELISA.
Immunoprecipitation (5 µg/ml). Immunohistochemistry on paraffin embedded sections (1 µg/ml, demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland). Western blotting (0.1 µg/ml, detects a 10 kDa protein representing S100-alpha from human muscle; S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane). |
| Specificity | SP5355P detects S100-alpha protein and shows no cross-reactivity with the S100-beta protein. Species: Bovine, human, mouse, porcine, and rat. |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Biomed. Res., 8(2): 119-125, 1987
2. Neurochem. Int., 6(4): 513-518, 1984 |
| Pictures | Figure 1 shows a Western blot of S100-alpha from human muscle extract. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP5355P S100A1 / Protein S100-A1 '.
