SP5385P c-myc Epitope Tag (EQKLISEEDL) antibody
see related secondary antibodiessee all 33 c-myc Epitope Tag products
0.1 ml / US$ 355
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti c-myc Epitope Tag (EQKLISEEDL)
Product Description
Rabbit anti c-myc Epitope Tag (EQKLISEEDL) , Presentation: Aff - Purified. Product is tested for Immunofluorescence ( IF )
Properties
| Applications | Immunofluorescence ( IF ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP5385P |
| Quantity | 0.1 ml |
| Price | US$ 355 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP5385P.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), 6-histidine (6X-His), green fluorescent protein (GFP), c-myc and others.
The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation. In addition, deregulated expression of c-Myc induces apoptosis in different cell types, with c-myc requiring p53 for apoptosis in many cell types. This fact indicates heterogeneous mechanisms for c-myc-induced apoptosis. |
| Immunogen | Synthetic peptide corresponding to amino acid residues 410-419 of human cMyc protein. AA Sequence: C E(410) Q K L I S E E D L(419) |
| Format | State: Liquid purified IgG fraction Purification: Epitope affinity chromatography BufferSystem: PBS containing 1.0 mg/ml BSA and 0.05 % sodium azide |
| Applications | Immunofluorescence (1/100). |
| Specificity | The antibody detects the presence of expressed cMyc-tagged proteins in transfected mammalian cells. |
| Add. Information | SP5385P immunizing peptide (SP5385CP) is available for use in neutralization and control experiments.
|
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | J Physiol 559.3, 849-862 Sept 2004.
Hum. Mol. Genet., Vol 12: 2519-2532, Oct 2003. |
| Pictures | Immunolocalization of c-Myc in transfected mammalian cells using SP5385P |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP5385P c-myc Epitope Tag (EQKLISEEDL)'.
