SP5431P DFF45 / ICAD / DFFA antibody
see related secondary antibodiessee all 40 DFF45 / ICAD / DFFA products
0.1 mg / US$ 535
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti DFF45 / ICAD / DFFA
Synonyms
Inhibitor of CAD, DFF-45, DFF1, , DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
Product review
Please read our product review about DFF45 / ICAD / DFFA: Antibodies to DNA Fragmentation Factor: DFF40 (CAD) and DFF45 (ICAD).
Product Description
Rabbit anti DFF45 / ICAD / DFFA , Presentation: Purified. Product is tested for Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | SP5431P |
| Swiss Prot. No. | O00273 |
| Quantity | 0.1 mg |
| Price | US$ 535 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP5431P.pdf |
Datasheet Extract
| Alternate names | ICAD, DFF45, DFFA |
| Concentration | 0.5 mg/ml |
| Background | Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase.
A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. A mouse homologue of human DFF was identified as inhibitor of caspase activated deoxyribonuclease (ICAD). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death. |
| Immunogen | Synthetic Peptide: K(313) A S P P G D L Q N P K R A R Q D P T(331) corresponding to amino acid residues 313-331 from human DFF40. Swiss Num.: O00273 Remarks: SP5431P immunizing peptide SP5431P immunizing peptide (SP5431CP) is available for use in Neutralization and Control experiments. |
| Format | State: Liquid purified IgG fraction. Purification: Affinity chromatography. BufferSystem: PBS containing 0.02% sodium azide as preservative. |
| Applications | Western Blot: 1/1500, detects an ~45 kDa protein representing DFF45 from HeLa cell lysate.
Immunoprecipitation. |
| Specificity | SP5431P detects DNA fragmentation factor 45 (DFF45) from human cells.
This antibody detects both the cleaved and non-cleaved forms of human DFF45. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Cell, 89: 175-184, 1997.
2. Nature, 391: 43-50, 1998. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP5431P DFF45 / ICAD / DFFA'.
