SP6224P DFF40 / CAD / DFFB (205-222) antibody
see related secondary antibodiessee all 31 DFF40 / CAD / DFFB products
50 µg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti DFF40 / CAD / DFFB
Synonyms
Caspase-activated DNase, DFF-40, DFF2, CPAN, , DNA fragmentation factor subunit beta, DNA fragmentation factor 40 kDa subunit, Caspase-activated deoxyribonuclease, Caspase-activated DNase, Caspase-activated nuclease
Product review
Please read our product review about DFF40 / CAD / DFFB : Antibodies to DNA Fragmentation Factor: DFF40 (CAD) and DFF45 (ICAD).
Product Description
Rabbit anti DFF40 / CAD / DFFB , Presentation: Aff - Purified. Product is tested for Frozen sections ( C ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Western blot / Immunoblot ( WB ) |
| Reactivity | Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP6224P |
| Swiss Prot. No. | O54788 |
| Quantity | 50 µg |
| Price | US$ 325 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP6224P.pdf |
Datasheet Extract
| Concentration | 0.2 mg/ml |
| Background | Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals cause the final degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1, 2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei (1-4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death. |
| Immunogen | Peptide corresponding to 17 amino acids near the center of murine CAD Swiss Num.: O54788 |
| Format | State: Liquid Ig fraction Purification: Affinity chromatography BufferSystem: 1x PBS containing 0.05 % Sodium Azide as preservative |
| Applications | Western Blot: 0.5 - 1 µg/ml.
Immunohistochemistry: 2 µg/ml. Positive Control: Murine kidney tissue lysate. |
| Specificity | This antibody reacts with CAD at the molecular weight of 40 kDa. Species: Mouse, rat. |
| Storage | Store at 2-8 °C.
Shelf life: one year from despatch. |
| References | 1. Enari M. et al. Nature 391, 43 (1998).
2. Sakahira H. et al. Nature 391, 96 (1998). 3. Liu X. et al. Proc. Natl. Acad. Sci. USA 95, 8461 (1998). 4. Halenbeck R. et al. Curr. Biol. 8,537 (1998). 5. Liu, X. el al. Cell 89, 175 (1997). |
| Pictures | Western blot analysis of CAD in murine kidney tissue lysate with anti- CAD at 2 µg/ml. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP6224P DFF40 / CAD / DFFB '.
