SP6226P DFF40 / CAD / DFFB (3-18) antibody
see related secondary antibodiessee all 31 DFF40 / CAD / DFFB products
50 µg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti DFF40 / CAD / DFFB
Synonyms
Caspase-activated DNase, DFF-40, DFF2, CPAN, , DNA fragmentation factor subunit beta, DNA fragmentation factor 40 kDa subunit, Caspase-activated deoxyribonuclease, Caspase-activated DNase, Caspase-activated nuclease
Product review
Please read our product review about DFF40 / CAD / DFFB : Antibodies to DNA Fragmentation Factor: DFF40 (CAD) and DFF45 (ICAD).
Product Description
Rabbit anti DFF40 / CAD / DFFB , Presentation: Aff - Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP6226P |
| Swiss Prot. No. | O76075 |
| Quantity | 50 µg |
| Price | US$ 325 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP6226P.pdf |
Datasheet Extract
| Alternate names | CPAN, hCAD |
| Concentration | 0.2 mg/ml |
| Background | Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Death domain containing adapter molecules and members of the caspase family of proteases transduce cell death signals. These death signals cause the final degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was recently identified and designated caspase activated deoxyribonuclease (CAD) (1, 2). The human homologue of mouse CAD was independently identified by three groups and termed CPAN, DFF40, and human CAD, respectively (3-5). DFF45/ICAD is the inhibitory protein of DFF40/CAD (1, 2, 6) and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death. |
| Immunogen | A peptide corresponding to amino acids 3 to 18 of human DFF40. Swiss Num.: O76075 |
| Format | State: Liquid Ig fraction Purification: Affinity chromatography BufferSystem: 1x PBS containing 0.05 % sodium azide |
| Applications | Western blot: (0.25-1.0 µg/ml, detects a band of 40 kDa).
Positive Control: K562, Jurkat whole cell lysate. |
| Specificity | This antibody is specific for DFF40. Species: Human, Mouse. |
| Storage | Store the antibody undiluted at 4 - 8°C.
Shelf life: one year from despatch. |
| References | 1. Enari M. et al. Nature 391, 43 (1998).
2. Sakahira H. et al. Nature 391, 96 (1998). 3. Halenbeck R. et al. Curr Biol 8, 537 (1998). 4. Liu X. et al. Proc. Natl. Acad. Sci. USA 95, 8461 (1998). 5. Mukae N. et al. Proc. Natl. Acad. Sci. USA 95, 9123 (1998). 6. Liu X. et al. Cell 1997; 89:175-84. |
| Pictures | Western blot analysis of DFF40 in Jurkat (J) and K562 (K) whole cell lysate with anti-DFF40 at 1 µg/ml |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP6226P DFF40 / CAD / DFFB '.
