SP6230P DFF45 / DFF35 antibody
see related secondary antibodies
50 µg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti DFF45 / DFF35
Synonyms
Inhibitor of CAD, DFF-45, DFF1, , DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
Product review
Please read our product review about DFF45 / DFF35: Antibodies to DNA Fragmentation Factor: DFF40 (CAD) and DFF45 (ICAD).
Product Description
Rabbit anti DFF45 / DFF35 , Presentation: Aff - Purified. Product is tested for Frozen sections ( C ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP6230P |
| Swiss Prot. No. | O00273 |
| Quantity | 50 µg |
| Price | US$ 325 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP6230P.pdf |
Datasheet Extract
| Concentration | 0.2 mg/ml |
| Background | Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain contained adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently, that was cleaved by caspase-3 during apoptosis (1). Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively, (2,4). Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2-5). Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death. |
| Immunogen | A peptide corresponding to amino acids 2 to 21 of human DFF45 (1). Swiss Num.: O00273 |
| Format | State: Liquid purified Ig fraction BufferSystem: 1x PBS containing 0.05 % sodium azide |
| Applications | Western blot (1:1000 - 1/2000, a band of 35 and 45 kDa can be detected).
Immunhistochemistry. Positive Control: Jurkat whole cell lysate. |
| Specificity | This antibody is specific for DFF45 and DFF35. Species: Human, Mouse. |
| Storage | Store at 2 - 8 °C.
Shelf life: one year from despatch. |
| References | 1. Liu X. et al. Cell 89, 175 (1997).
2. Enari M. et al. Nature 391, 43 (1998). 3. Sakahira H. et al. Nature 391, 96 (1998). 4. Gu J. et al. J Biol Chem 274, 20759 (1999). 5. Liu X. et al. Proc Natl Acad Sci USA 95, 8461 (1998). 6. Mukae, N. et al. Proc Natl Acad Sci USA 95, 9123 (1998). |
| Pictures | Western blot analysis of DFF45/35 in HeLa, (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with anti-DFF45 at 1:1000 dilution. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP6230P DFF45 / DFF35'.
