SP6273P FLIP / I-FLICE / Casper (Long/a) (C-Term) antibody
see related secondary antibodiessee all 55 FLIP / I-FLICE / Casper products
50 µg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti FLIP / I-FLICE / Casper (Long/a)
Synonyms
CASH, CASP8AP1, CLARP, FLAME-1, MRIT, USURPIN, c-FLIP, CFLAR, , CASP8 and FADD-like apoptosis regulator,Cellular FLICE-like inhibitory protein, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, MACH-related inducer of toxicity, Inhibitor of FLICE, FADD-like antiapoptotic molecule 1
Product review
Please read our product review about FLIP / I-FLICE / Casper (Long/a) : Antibodies to FLIP/I-FLICE/Casper.
Product Description
Rabbit anti FLIP / I-FLICE / Casper (Long/a) , Presentation: Aff - Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Mouse ( Ms ), Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | SP6273P |
| Swiss Prot. No. | O15519 |
| Quantity | 50 µg |
| Price | US$ 325 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP6273P.pdf |
Datasheet Extract
| Alternate names | Casper, I-FLICE, FLAME-1, CASH, CLARP |
| Concentration | 0.2 mg/ml |
| Background | Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family, such as caspases-8 (FLICE) and -10 (FLICE2). FLICE-inhibitory proteins were identified in viruses and humans and designated v-FLIPs and FLIP, respectively (1, 2). The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP (3-7). FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIP α, β, γ, and δ (8). |
| Immunogen | Peptide corresponding to amino acids 447 to 464 at C-terminus of human FLIPα/FLIPL form2. Swiss Num.: O15519 Remarks: Peptide available as SP6273CP. |
| Format | State: Liquid purified Ig fraction BufferSystem: 1x PBS containing 0.05% sodium azide |
| Applications | Western blot: 0.5-1.0 µg/ml.
HeLa whole cell lysate can be used as positive control and a 55 kDa band can be detected. |
| Specificity | This antibody is specific for FLIPα (CT). Species: Human, Mouse. |
| Storage | Store the antibody at 4-8°C for one month or (in aliquots) at -20 °C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Thome, M. et al. Nature 386, 517 (1997).
2. Irmler, M. et al. Nature 388, 190 (1997). 3. Shu, HB. et al. Immunity 6, 751 (1997). 4. Hu, S. et al. J. Biol. Chem. 272, 17255 (1997). 5. Srinivasula, SM. et al. J. Biol. Chem. 272, 18542 (1997). 6. Goltsev, YV. et al. J. Biol. Chem. 272, 19641 (1997). 7. Inohara, N. et al. Proc. Natl. Acad. Sci. USA 94, 10717 (1997) 8. Wallach, D. et al. Nature 388, 123 (1997). |
| Pictures | Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with anti-FLIP at 0.5 µg/ml |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP6273P FLIP / I-FLICE / Casper (Long/a) '.
