SP7024P Bcl-3 antibody
see related secondary antibodiessee all 9 Bcl-3 products
0.1 mg / US$ 405
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Bcl-3
Synonyms
BCL3, BCL4, D19S37, B-Cell Lymphoma 3-encoded Protein
Product Description
Rabbit anti Bcl-3 , Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | SP7024P |
| Swiss Prot. No. | P20749 |
| Quantity | 0.1 mg |
| Price | US$ 405 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP7024P.pdf |
Datasheet Extract
| Concentration | 0.5 mg/ml |
| Background | NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). The proto-oncoprotein Bcl-3 is a member of the IkB family and is present predominantly in the nucleus. IkBa and Bcl-3 are similar in the central ankyrin repeat domains, where as they differ at the N- and -C terminus. These differences may explain the differential binding of IkBa and Bcl-3. Two molecules of Bcl-3 can associate with NF-kB, where as only one molecule of IkBa bind to NF-kB. BCL-3 mRNA and protein expression are induced by NF-kB-activating agents. Down-regulation of BCL-3 induction results in prolonged, enhanced NF-kB binding and increased NF-kB-dependent transcription. Several cytokines, such as, TNFa or IL-1b, and mitogens as phorbolester (PMA) trigger rapid formation of Bcl-3-p50 complexes with the same kinetics as activation of p50-p65 complexes. |
| Immunogen | Synthetic peptides corresponding to amino acids 2-13 and 107-120 of human Bcl-3. Swiss Num.: P20749 |
| Format | State: Liquid Ig fraction Purification: Protein G chromatography BufferSystem: PBS containing 0.02 % sodium azide and 0.05 % BSA |
| Applications | Western blot: 0.5 - 1.0 µg/ml. Recommended positive control: Jurkat. In Jurkat, a 45 kDa band is observed. |
| Specificity | This antibody is specific for Bcl-3. Species: Human, Mouse, Rat. |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Verma, I.M., Stevenson, J.K., Schwarz, E.M., Van Antwerp, D. & Miyamoto, S. Genes. Dev. 9: 2723-2735, (1995).
2. Michel F, Soler-Lopez M, Petosa C, Cramer P, Siebenlist U, Muller CW. EMBO J 20(22): 6180-6190 (2001). 3. Brasier AR, Lu M, Hai T, Lu Y, Boldogh I. J Biol Chem 276(34): 32080-32093 (2001). 4. Hunter RB, Stevenson E, Koncarevic A, Mitchell-Felton H, Essig DA, Kandarian SC. FASEB J 16(6): 529-538 (2002). |
| Pictures | Western blot analysis of Bcl-3 in Jurkat cells using SP7024P at 0.5 µg/ml.
|
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP7024P Bcl-3 '.
