SP7029P DFF40 / CAD / DFFB (205-222) antibody
see related secondary antibodiessee all 31 DFF40 / CAD / DFFB products
0.1 mg / US$ 405
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti DFF40 / CAD / DFFB
Synonyms
Caspase-activated DNase, DFF-40, DFF2, CPAN, , DNA fragmentation factor subunit beta, DNA fragmentation factor 40 kDa subunit, Caspase-activated deoxyribonuclease, Caspase-activated DNase, Caspase-activated nuclease
Product review
Please read our product review about DFF40 / CAD / DFFB : Antibodies to DNA Fragmentation Factor: DFF40 (CAD) and DFF45 (ICAD).
Product Description
Rabbit anti DFF40 / CAD / DFFB , Presentation: Purified. Product is tested for Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Mouse ( Ms ), Genua Pig ( GP ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | SP7029P |
| Swiss Prot. No. | O54788 |
| Quantity | 0.1 mg |
| Price | US$ 405 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP7029P.pdf |
Datasheet Extract
| Concentration | 0.5 mg/ml |
| Background | Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei1 (4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death. |
| Immunogen | Peptide corresponding to amino acids 205 to 222 of mouse CAD (1) Swiss Num.: O54788 |
| Format | State: Liquid Ig fraction Purification: Protein G chromatography BufferSystem: PBS containing 0.02 % sodium azide |
| Applications | IHC (paraffin): 1:1000 (see Watanabe et al. for details).
Western blot: 1/500 - 1/1000. Recommended positive control: mouse lung. A 40 kDa band should be observed. |
| Specificity | This antibody DFF40 (205-222). Species: Mouse, Guinea pig. |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | 1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50.
2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99. 3. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6. 4. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40. 5. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-18. Product Citations 1. Expression of caspase-activated deoxyribonuclease (CAD) and caspase 3 (CPP32) in the hydropic cochlea of guinea pigs-second report. Kenichi Watanabe, Shunichi Tomiyama, Ken Jinnouchi and Toshiaki Yagi. Eur Arch Otorhinolaryngol, 259: 257-261 (2002). 2. Over Expression Of Inhibitor Of Caspase 3 Activated Deoxyribonuclease In Human Renal Cell Carcinoma Cells Enhances Their Resistance To Cytotoxic Chemotherapy In Vivo. Shoji Hara, Hideaki Miyake, Soichi Arakawa, Sadao Kamidono And Isao Hara. The Journal Of Urology Vol. 166, 2491â??2494 (2001). 3. Expression of caspase-activated deoxyribonuclease (CAD) and caspase 3 (CPP32) in the cochlea of cisplatin (CDDP)-treated guinea pigs. K Watanabe, IS Jinnouchi, K Baba, and T Yagi. Auris Nasus Larynx. 30: 219-225 (2003). IHC (Paraffin). |
| Pictures | Western blot analysis of 50 µg of murine lung tissue lysate with anti-CAD (I17) at 1:500 dilution. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP7029P DFF40 / CAD / DFFB '.
