SP7059P DFF45 / ICAD / DFFA antibody
see related secondary antibodiessee all 40 DFF45 / ICAD / DFFA products
0.1 mg / US$ 405
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti DFF45 / ICAD / DFFA
Synonyms
Inhibitor of CAD, DFF-45, DFF1, , DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
Product review
Please read our product review about DFF45 / ICAD / DFFA: Antibodies to DNA Fragmentation Factor: DFF40 (CAD) and DFF45 (ICAD).
Product Description
Rabbit anti DFF45 / ICAD / DFFA , Presentation: Purified. Product is tested for Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | SP7059P |
| Swiss Prot. No. | O00273 |
| Quantity | 0.1 mg |
| Price | US$ 405 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP7059P.pdf |
Datasheet Extract
| Concentration | 0.5 mg/ml |
| Background | Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (1). A mouse homologue of human DFF was identified as a DNase inhibitor and designated ICAD (2,3). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2,3). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death (4). |
| Immunogen | Peptide corresponding to amino acids 313-331 at the C-terminus of human DFF1 Swiss Num.: O00273 |
| Format | State: Liquid Ig fraction Purification: Protein G affinity chromatography BufferSystem: PBS containing 0.02% sodium azide |
| Applications | Immunoprecipiation.
Western blot analysis (1:1000 -1:2000): A 45 kDa band should be observed. Positive control: HeLa cells. |
| Specificity | The antibody reacts with DFF. Species: Human. |
| Storage | Store the antibody at 2-8°C.
Shelf life: one year from despatch. |
| References | 1. Wyllie A. Apoptosis. An endonuclease at last. Nature 1998; 391:20-21.
2. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997; 89:175-184. 3. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998; 391:43-50. 4. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99. |
| Pictures | Western blot analysis of 50 µg of total cell lysate from HeLa cells with anti-DFF (CT) at 1:2000 dilution.
|
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP7059P DFF45 / ICAD / DFFA'.
