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Western blot analysis of IkappaBz using antibody SP7353P at 3 µg/ml in mouse RAW cell lysate.

 

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SP7353P  I Kappa B zeta (IKBz) antibody

see related secondary antibodies
0.1 mg / US$ 425
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti I Kappa B zeta (IKBz)

Product Description

Rabbit anti I Kappa B zeta (IKBz) , Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB )

Properties

ApplicationsWestern blot / Immunoblot ( WB )
ReactivityMouse ( Ms ), Human ( Hu ), Rat ( Rt ), Monkey ( Mky ), Bovine ( Bov )
PresentationPurified
HostRabbit
Catalog NumberSP7353P
Quantity0.1 mg
PriceUS$ 425
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
SP7353P.pdf

Datasheet Extract

Concentration0.5 mg/ml
BackgroundIkappaBzeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein that is highly homologous to the IkappaB family member Bcl-3. Transcription of IkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.
ImmunogenAmino acids 684-699 and 285-298 of mouse IkBz protein
AA Sequence: RKGADPSTRNLENEQP (684-699), YQYSPQTQNMQQPP (258-298)
Format
State: Liquid Ig fraction
Purification: Protein G affinity chromatography
BufferSystem: PBS, containing 0.2% gelatin and 0.05% sodium azide
ApplicationsWestern Blot: 1 - 3 µg/ml.
Recommended Positive Control: RAW.
SpecificityThis antibody reacts with mouse IKappaBz. Reactivity with human, monkey (chimpanzee), cow and rat can be predicted from 100% amino acid sequence homology with the immunogen; the sequence is 93% homologous in chicken.
Species: Mouse, human, rat, monkey, bovine.
StorageStore at 4°C, stable for 6 months. For long term storage, store at -20°C.
Avoid repeated freezing and thawing. Shelf life: one year from despatch.
References1. Kitamura H, Matsushita Y, Iwanaga T, Mori K, Kanehira K, Fujikura D, Morimatsu M, Saito M. Arch Histol Cytol. 66(1): 53-62 (2003).
2. Yamamoto M, Yamazaki S, Uematsu S, Sato S, et al. Nature. 430(6996):218-222 (2004).
3. Yamazaki S, Muta T, Takeshige K. J Biol Chem. 276(29): 27657-27662 (2001).
PicturesWestern blot analysis of IkappaBz using antibody SP7353P at 3 µg/ml in mouse RAW cell lysate.

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1364B Rabbit IgG (H&L)  
  Goat Biotin   2 mg / US$ 295
   
R1364F Rabbit IgG (H&L)  
  Goat FITC   2 mg / US$ 285
   
R1364T Rabbit IgG (H&L)  
  Goat TRITC   2 mg / US$ 285
   
R1364TR Rabbit IgG (H&L)  
  Goat Texas Red   2 mg / US$ 295
   
R1364HRP Rabbit IgG (H&L)  
  Goat HRP   2 mg / US$ 295
   
R1364AP Rabbit IgG (H&L)  
  Goat AP   1 mg / US$ 325
   
R1458C2 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy2   1 mg / US$ 445
   
R1458C3 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3   1 mg / US$ 445
   
R1458C35 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy3.5   1 mg / US$ 445
   
R1458C5 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5   1 mg / US$ 445
   
R1458C55 Rabbit IgG (H&L) multi-species ads.  
  Goat Cy5.5   1 mg / US$ 445
   
R1427R Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / US$ 455
   

Click here to see all secondary antibodies for 'SP7353P I Kappa B zeta (IKBz)'.