SP7364P SAE2 antibody
see related secondary antibodiessee all 7 SAE2 products
0.1 mg / US$ 425
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti SAE2
Product Description
Rabbit anti SAE2 , Presentation: Purified. Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Purified |
| Host | Rabbit |
| Catalog Number | SP7364P |
| Quantity | 0.1 mg |
| Price | US$ 425 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  SP7364P.pdf |
Datasheet Extract
| Concentration | 0.5 mg/ml |
| Background | Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes. The conjugation of SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1/SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase. |
| Immunogen | Synthetic peptide within amino acid residues 160-210 of human SAE2 |
| Format | State: Liquid Ig fraction Purification: Protein G Chromatography BufferSystem: PBS containing 0.2% gelatin and 0.05% sodium azide |
| Applications | Western blot: 0.5 - 2.0 µg/ml.
Recommended positive control: HeLa cell lysate. |
| Specificity | This antibody is specific for SAE2. Species: Human, Mouse, Rat. |
| Storage | Store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Tatham MH, Kim S, Jaffray E, Song J, Chen Y, Hay RT. Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection. Nat Struct Mol Biol. 2004 Dec 19; [Epub ahead of print].
2. Identification of the Enzyme Required for Activation of the Small Ubiquitin-like Protein SUMO-1.Joana M. P.Desterro, Manuel S. Rodriguez, Graham D. Kemp, and Ronald T. Hay. J Biol Chem, Vol. 274 (15): 10618-10624 (1999). Product Citations 1. NXP-2 association with SUMO-2 depends on lysines required for transcriptional repression. Adam Rosendorff, Shuhei Sakakibara, Sixin Lu, Elliott Kieff, Yan Xuan, Alessandra DiBacco, Yujiang Shi, Yang Shi, and Grace Gill. PNAS, Mar 2006; 10.1073/pnas.0601066103. |
| Pictures | Western bot analysis of SAE2 in HeLa cell lysate using SP7364P at 1.0 µg/ml.
|
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'SP7364P SAE2'.
