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Product Description for Collagen type I
Properties for Collagen type I
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 0.1 mg |
| Reactivity | Human |
| Presentation | Purified |
| Applications | Frozen sections, Western blot / Immunoblot |
| Isotype | IgG2a |
| Host | Mouse |
| Clone | I-8H5 |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen |
| Swiss Prot Num | P02452 |
| Product Type | Antibodies |
| Recommended Isotype Control | AM03096PU-N |
| Storage | Store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | Mayne, R. et al. Isolation and partial characterization of basement membrane-link collagens from bovine thoracic aorta. Artery, 7. 262-280, 1980 Muragaki, Y. et al., Experimental study on alteration of collagen metabolism in liver fibrosis. J. Wakayama Med. Soc., 36. 195-208, 1985 (in Japanese) Kumagai, J. et al. (1994) Immunohistochemical distribution of type I, II and III collagens in the rabbit supraspinatus tendon insertion. J. Anat. 185:279-284 Marijianowski, M.M.H. et al. Dilated cardiomyopathy is associated with an increase in the type I/type III collagen ration: A quantitative assessment. J. Am. College Cardiol., 25. 1263-1272, 1995 |
| Format | Reconstitution: Reconstitute in 0.5 ml distilled water. |
| State: Lyophilized | |
| Purification: Protein A affinity purified immunoglobulin fraction | |
| Buffer System: 0.1 M Na-Phosphate buffer, pH 7.0 containing 0.5% protease-free bovine serum albumin and Kathon as a preservative | |
| Specificity | The antibody specifically reacts with human Collagen type I and cross-reacts with rabbit Collagen type I. |
| Application | Immunohistochemistry on frozen sections (1-10 µg/ml). Western blotting (4 µg/ml, non-denaturing, non-reducing conditions, use 4-6% PAGE, bands are observed at 120 kD and 300 kD). |
| Background | Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. |
Secondary Antibodies for Collagen type I
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 190 Euro | |||||
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170 Euro | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160 Euro | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 180 Euro | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170 Euro | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment | ||||||
| Rabbit | PE | 1 ml / 290 Euro | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment | ||||||
| Goat | PE | 1 ml / 290 Euro | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 270 Euro | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||
