You have no items in your shopping cart.
Product Description for TIMP3
Properties for TIMP3
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 0.1 mg |
| Reactivity | Human |
| Presentation | Purified |
| Applications | Frozen sections, Paraffin Sections, Western blot / Immunoblot |
| Isotype | IgG1 |
| Host | Mouse |
| Clone | 136-13H4 |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | TIMP-3, Tissue inhibitor of metalloproteinases 3, Metalloproteinase inhibitor 3, MIG-5, Review 002 |
| Swiss Prot Num | P35625 |
| Immunogen | Oligopeptide of residues 170 - 188 of human Tissue Inhibitor of Metalloproteinases-3 (hTIMP-3) |
| Product Type | Antibodies |
| Recommended Isotype Control | SM10P (for use in human samples), AM03095PU-N |
| Storage | Store at 2-8°C for up to one month, aliquot and store at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | Apte, S.S. et al., Genomics, 19. 86-90, 1994. Wilde, C.G. et al., DNA Cell Biol., 13. 711-718, 1994. Apte, S.S. et al., J. Biol. Chem., 270. 14313-14318, 1995. Fariss, R.N. et al., Am. J. Pathol., 150. 323-328, 1997. |
| Format | State: Liquid Ig fraction |
| Purification: Purified immunoglobulin fraction | |
| Buffer System: 0.1 M Na-Phosphate buffer, pH 7.0 containing 0.5% protease-free bovine serum albumin and 0.09% sodium azide as preservative | |
| Specificity | The antibody specifically reacts with human TIMP-3 and cross-reacts with rabbit TIMP-3, but not with hTIMP-1 and hTIMP-2. |
| Application | Western blotting. Immunohistochemistry on frozen or paraffin sections (10 µg/ml). |
| Background | The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tightly bound inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knotlike structures. The N terminal region is necessary for inhibitory activity. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved to produce the mature protein. The C terminal regions are divergent and enhance the inhibition selectivity and binding efficiency. Although the TIMP proteins share high homology, following secretion they are localized extracellularly either in soluble form (TIMP1, TIMP2, and TIMP4) or bound to extracellular matrix components (TIMP3). The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL beta. A marked cell type specificity is a hallmark of both MMP and TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinase 3 (TIMP3) was first purified from chicken embryo fibroblasts and identified as ChIMP3. The human homologue of TIMP3, was originally detected as an inducible serum protein in WI-38 fibroblasts. The TIMP3 localization differs from that of the other three TIMPs, and is thought to be primarily deposited into the extracellular matrix (ECM). TIMP3 is insoluble, binds to the ECM associated with a variety of cell types, and is widely distributed throughout the body. TIMP3 shows 30% amino acid homology with TIMP1 and 38% homology with TIMP2. TIMP3 has been shown to promote the detachment of transformed cells from ECM and to accelerate morphological changes associated with cell transformation. Furthermore, up regulation of TIMP 3 has been associated with a block in the G1 phase of the cell cycle during differentiation of HL60 leukemia cells. The human TIMP3 gene has the chromosomal location of 22q12-22q13. TIMP3 mRNA is highly expressed in placenta but is also found in the heart, kidney, lung, pancreas, uterus and skeletal muscle with low levels in the brain. In endometrium, TIMP3 is reported to be expressed in luminal epithelium, glands, stroma, endothelial cells and vascular smooth muscle cells. TIMP3 is also reported to be expressed by fibroblast-like cells in ulcerated intestinal wall. |
| Concentration | 0.5 mg/ml |
| Review | r002 Antibodies to TIMP Protein Family |
Secondary Antibodies for TIMP3
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 190 Euro | |||||
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170 Euro | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160 Euro | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 180 Euro | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170 Euro | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment | ||||||
| Rabbit | PE | 1 ml / 290 Euro | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment | ||||||
| Goat | PE | 1 ml / 290 Euro | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 270 Euro | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||
