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Product Description for Ki-67
Properties for Ki-67
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 2 ml |
| Reactivity | Human |
| Presentation | Supernatant |
| Applications | Frozen sections, Flow cytometry, Immunofluorescence |
| Isotype | IgG1 |
| Host | Mouse |
| Clone | Ki67 |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | Ki67, MKI67, FocusOn056 |
| Swiss Prot Num | P46013 |
| Immunogen | Human tumour cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag 8.653 myeloma cell line. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Freeman, A.S. and Nadler, A.S. (1991) Immunologic markers in non-Hodgkin's lymphoma. Non-Hodgkin's Lymphoma 5: 871-889. 2. Gerdes, J. et al. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int. J. Cancer 31: 13-20. 3. Gerdes, J. et al. (1984) Cell cycle analysis of a cell-proliferation-associated human nuclear antigen defined by a monoclonal antibody Ki67. J. Immunol. 133: 1710-1715. 4. Gerdes, J. et al. (1987) Prognostic relevance of tumour-cell growth fraction in malignant non-Hodkin's lymphomas. Lancet. 11: 448. 5. Grogan, T.M. et al. (1988) Independant prognostic significance of a nuclear proliferation antigen in diffuse large cell lymphomas as determined by the monoclonal antibody Ki67. Blood 71: 1157-1160. 6. Grogan, T.M. and Miller, T.P. (1991) New biologic markers in non-Hodgkin's lymphomas. Non-Hodgkin's Lymphoma 5: 925-933. 7. Hall, P.A. et al. (1988) The prognostic value of Ki67 immunostaining in non-Hodgkin's lymphoma. J. Pathol. 154: 223-235. 8. Houmand, A. et al. (1992) Relevance of Ki67 expression in the classification of non-Hodgkin's lymphomas. Histopathology 20: 13-20. |
| Format | State: Liquid Tissue Culture Supernatant |
| Buffer System: 0.2M Tris/HCl, pH7.4 and 5-10% foetal calf serum containing 0.09% Sodium Azide | |
| Specificity | This antibody reacts with a nuclear antigen that is expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (Go) cells. |
| Application | Flow Cytometry: Neat - 1/5; Cell permeabilisation is required for this application; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on frozen sections: 1/10 - 1/50. Recommended Positive Control: Tonsil |
| Background | The monoclonal antibody is useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidinelabelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong. |
| Focuson | 056 Proliferation Marker Antibodies - FocusOn No.56 |
Secondary Antibodies for Ki-67
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 190 Euro | |||||
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170 Euro | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160 Euro | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 180 Euro | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170 Euro | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment | ||||||
| Rabbit | PE | 1 ml / 290 Euro | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment | ||||||
| Goat | PE | 1 ml / 290 Euro | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 270 Euro | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||
