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Product Description for Ki-67
Properties for Ki-67
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 1 ml |
| Reactivity | Human |
| Presentation | Aff - Purified |
| Applications | Paraffin Sections |
| Isotype | IgG |
| Host | Rabbit |
| Clone | C16-I |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | Ki67, MKI67, FocusOn056 |
| Swiss Prot Num | P46013 |
| Immunogen | Peptide derived from C-terminal region of human Ki-67. |
| Product Type | Antibodies |
| Recommended Isotype Control | PP501P |
| Storage | Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Protocols | IHC-P -PROTOCOL 1. Deparaffinize the section in 3 changes of xylene, 5 minutes each. 2. Wash the section in 96%, 80% and 70% benzyl alcohol for 5 minutes each 3. Rinse in distilled water 4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes 5. Rinse in distilled 6. For antigen retrieval: immerse the slide in Tris-EDTA buffer, pH 9.0, 0.05% Tween-20*, and incubate in water bath at 90ºC for 30 minutes 7. Remove the staining to room temperature and let the slide to cool (in TRIS-EDTA buffer, pH 9.0) for 15 minutes 8. Rinse in distilled water 9. Wash in 0.05 M Tris-HCl , pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes 10. Incubate the section with primary antibody diluted in buffer A at the dilution 1:100-400 for 1 hour in the closed wet chamber 11. Wash twice 5 minutes with buffer A 12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP – Peroxide – DAB) 13. Wash twice 5 minutes with buffer A 14. Apply of chromogen (DAB), 10 minutes 15. Rinse in water – 10 minutes 16. Stain in hematoxylin for 5 minutes 17. Wash in water – 10 minutes 18. Dehydrate the section in 2 changes of 96% benzyl alcohol for 5 minutes each 19. Wash the section in 2 changes of xylene for 2 minutes each 20. Mount the slide for observation. * EDTA Buffer (10mM Tris Base, 1 mM EDTA Solution, 0.05% Tween 20, pH 9.0): TRIS ------------------1.21 g EDTA ------------------0.37 g Distilled water -------1000 ml Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at 4°C for longer storage. |
| Format | State: Liquid purified Ig fraction. |
| Purification: Immunoaffinity Chromatography. | |
| Buffer System: 20 mM Tris-HCl, pH 8.0 containing 20 mg/ml BSA as stabilizer and 0.05% Sodium Azide as preservative. | |
| Specificity | This antibody recognises Ki-67. |
| Application | Immunohistochemistry on Paraffin Sections (1/100-1/200). |
| Background | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
| Focuson | 056 Proliferation Marker Antibodies - FocusOn No.56 |
Secondary Antibodies for Ki-67
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 190 Euro | |||||
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 160 Euro | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160 Euro | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170 Euro | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160 Euro | |||||
| R1427R | Rabbit IgG F(ab)2 Fragment | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1458C2 | Rabbit IgG (H&L) | ||||||
| Goat | Cy2 | 1 mg / 250 Euro | |||||
| R1458C3 | Rabbit IgG (H&L) | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1458C5 | Rabbit IgG (H&L) | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||
