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Product Description for Ki-67
Properties for Ki-67
| Manufacturer | Acris Antibodies GmbH |
| Quantity | 0.1 ml |
| Reactivity | Human, Rat |
| Presentation | Supernatant |
| Applications | Paraffin Sections, Western blot / Immunoblot |
| Host | Rabbit |
| Clone | SP6 |
| PDF datasheet | view  |
| Shipping to | Worldwide |
Datasheet Extract
| Synonyms | Ki67, MKI67, FocusOn056 |
| Swiss Prot Num | P46013 |
| Immunogen | A synthetic peptide from C-terminus of Human Ki-67. |
| Product Type | Antibodies |
| Immunogen | Swiss Prot Num: P46013 |
| Remarks: Epitope: C-terminus. | |
| Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
| Format | State: Liquid Tissue Culture Supernatant with 0.09% Sodium Azide as preservative. |
| Specificity | The antibody recognizes native Ki-67 antigen and recombinant fragments of the Ki-67 molecule (345 and 395 kD double band in immunoblotting of protein extracted from proliferating cells) associated with cell proliferation and found throughout the cell cycle (G1, S, G2, M-phases), and absent in resting cells (G0 phase). Cellular Localization: Nuclear. |
| Application | Western blot: 1/50 for 2 hrs at RT. Immunohistochemistry on Paraffin Sections: 1/200. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 minutes at RT. Positive Control: Tonsil. |
| Background | Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. |
| Species Reactivity | Tested: Human and Mouse. |
| Expected from sequence similarity:Rat. | |
| Focuson | 056 Proliferation Marker Antibodies - FocusOn No.56 |
Secondary Antibodies for Ki-67
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 190 Euro | |||||
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 160 Euro | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160 Euro | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170 Euro | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160 Euro | |||||
| R1427R | Rabbit IgG F(ab)2 Fragment | ||||||
| Donkey | PE | 1 ml / 290 Euro | |||||
| R1458C2 | Rabbit IgG (H&L) | ||||||
| Goat | Cy2 | 1 mg / 250 Euro | |||||
| R1458C3 | Rabbit IgG (H&L) | ||||||
| Goat | Cy3 | 1 mg / 250 Euro | |||||
| R1458C5 | Rabbit IgG (H&L) | ||||||
| Goat | Cy5 | 1 mg / 250 Euro | |||||
