14-3-3 protein epsilon - AP05568SU-N | acris-antibodies.com

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Product Description for 14-3-3 protein epsilon

Rabbit anti Bovine, Chicken, Human, Mouse, Rabbit, Rat, Sheep 14-3-3 protein epsilon.
Presentation: Serum
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for 14-3-3 protein epsilon

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	14-3-3E, FocusOn116, YWHAE
Presentation	Serum
Reactivity	Bov, Chk, Hu, Ms, Rb, Rt, Sh
Applications	E, P, WB
Clonality	Polyclonal
Host	Rabbit
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P62258 Immunogen: Synthetic peptide corresponding to acetylated N-terminal sequence of Sheep 14-3-3 epsilon AA Sequence: `Ac.MDDREDLVYQAKC` Genename: 7531 Remarks: Antiserum Preparation: Antisera to anti 14-3-3 epsilon N-terminus were raised by repeated immunisations of rabbits with highly purified antigen.
Antibody type	Ab
Application	ELISA. Western Blot: 1/3000. Detects a band of approximately 30kDa in HEK293 cell lysates. Immunohistochemistry on Paraffin Sections: 1/400. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Positive Control: Normal brain tissue.
Background	14-3-3 epsilon is a member of the 14-3-3 family which consists of 30 kDa proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G(2) checkpoint. During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene.
General Readings	Martin, H. et al. (1993) Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein. FEBS 331: 296-303. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162-172. Wang Z, Nesland JM, Suo Z, Trope CG, Holm R. The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3β and ε are independent prognostic factors for these tumors. PLoS One. 2011;6(9):e24843. doi: 10.1371/journal.pone.0024843. Epub 2011 Sep 15. PubMed PMID: 21935479.
Storage	Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Format	State: Liquid Serum containing 0.09% Sodium Azide as preservative Serum
Species Reactivity	Species reactivity (expected): Mammals. Species reactivity (tested): Bovine, Chicken, Human, Mouse, Rabbit, Rat, Sheep.
Specificity	Specificity: This antibody recognises the acetylated N-terminal of 14-3-3 epsilon. The antibody may not react with recombinant proteins that are not N-acetylated.
Gene ID	7531
FocusOn and Reviews	FocusOns: Antibody Panel to Proteins of the 14-3-3 family (14-3-3 beta, 14-3-3 gamma, 14-3-3 epsilon, 14-3-3 zeta, 14-3-3 theta, 14-3-3 sigma, 14-3-3 eta) - FocusOn 116
Targets	14-3-3 protein epsilon antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for 14-3-3 protein epsilon (5 products)

Catalog No.	Species	Pres.	Purity		Source
AR09100PU-L	14-3-3 protein epsilon (1-255)
	Human	Purified	≥ 95		E. coli		0.5 mg / €1,000.00
							Acris Antibodies GmbH
AR09100PU-N	14-3-3 protein epsilon (1-255)
	Human	Purified	≥ 95		E. coli		0.1 mg / €370.00
							Acris Antibodies GmbH
NBC1-18392	14-3-3 protein epsilon
							0.1 mg / €390.00
							Novus Biologicals Inc.
H00007531-P01-10	14-3-3 protein epsilon
	Human				in vitro transl.		10 µg / €350.00
							Abnova Taiwan Corp.
H00007531-P01-25	14-3-3 protein epsilon
	Human				in vitro transl.		25 µg / €500.00
							Abnova Taiwan Corp.

Recommended Secondary Antibodies for 14-3-3 protein epsilon (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat		AP
							Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat		Biotin
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R1364F	Rabbit IgG (H+L chain) antibody
	Goat		FITC
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R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat		HRP
							Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat		TRITC
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R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey		PE
							Acris Antibodies GmbH

Click here to see all secondary antibodies for 14-3-3 protein epsilon

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