14-3-3 protein sigma / SFN

Product Description for 14-3-3 protein sigma / SFN

Goat anti Canine, Human, Mouse, Rat 14-3-3 protein sigma / SFN.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for 14-3-3 protein sigma / SFN

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	Epithelial cell marker protein 1, FocusOn116, HME1, Stratifin
Presentation	Aff - Purified
Reactivity	Can, Hu, Ms, Rt
Applications	P, WB
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P31947 Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_006133.1. AA Sequence: `C-DLHTLSEDSYKDST` Genename: 2810
Antibody type	Ab
Application	Peptide ELISA: 1/64000. Western Blot: 0.1-0.3 µg/ml. Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Immunohistochemistry on Paraffin Sections: 2-3 µg/ml. In paraffin embedded Human Skin, this antibody shows strong nuclear staining in selected cells of the basal layer.
Background	Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. As 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. 14-3-3 sigma may be required for Raf-1 kinase activity and phosphorylation, among many other functions.
Concentration	0.5 mg/ml
General Readings	Lodygin D, Hermeking H. The role of epigenetic inactivation of 14-3-3sigma in human cancer. Cell Res. 2005 Apr;15(4):237-46. PubMed PMID: 15857578.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide. Buffer System: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Liquid purified Ig fraction. Aff - Purified
Species Reactivity	Species reactivity (expected): Mouse, Rat, Canine (Dog) and Porcine. Species reactivity (tested): Human.
Specificity	Specificity: This antibody recognizes 4-3-3 sigma/Stratifin.
Gene ID	2810
FocusOn and Reviews	FocusOns: Antibody Panel to Proteins of the 14-3-3 family (14-3-3 beta, 14-3-3 gamma, 14-3-3 epsilon, 14-3-3 zeta, 14-3-3 theta, 14-3-3 sigma, 14-3-3 eta) - FocusOn 116
Targets	14-3-3 protein sigma / SFN antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for 14-3-3 protein sigma / SFN (7 products)

Catalog No.	Species	Pres.	Purity	Source
AR09318PU-L	14-3-3 protein sigma / SFN (1-248)
	Human	Purified	> 95 %	E. coli	0.5 mg / €670.00
					Acris Antibodies GmbH
AR09318PU-N	14-3-3 protein sigma / SFN (1-248)
	Human	Purified	> 95 %	E. coli	0.1 mg / €250.00
					Acris Antibodies GmbH
NBP1-30172	14-3-3 protein sigma / SFN
					0.1 mg / €310.00
					Novus Biologicals Inc.
H00002810-P01-10	14-3-3 protein sigma / SFN
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00002810-P01-25	14-3-3 protein sigma / SFN
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00002810-Q01-10	14-3-3 protein sigma / SFN
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00002810-Q01-25	14-3-3 protein sigma / SFN
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for 14-3-3 protein sigma / SFN (1 products)

Catalog No.	Species
NBL1-15882	14-3-3 sigma Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for 14-3-3 protein sigma / SFN (6 products)

Catalog No.	Host	Pres.
R1317AP	Goat IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1317B	Goat IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1317F	Goat IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1317HRP	Goat IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1317T	Goat IgG (H+L chain) antibody
	Rabbit	TRITC
			Acris Antibodies GmbH
R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH