Product Description for 14-3-3 protein sigma / SFN
Mouse anti Human, Rat 14-3-3 protein sigma / SFN.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.
Properties for 14-3-3 protein sigma / SFN
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Epithelial cell marker protein 1, FocusOn116, HME1, Stratifin |
| Presentation | Purified |
| Reactivity | Hu, Rt |
| Applications | C, E, F, IP, WB |
| Clonality | Polyclonal |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Add. information | HT Cells, skin |
| Application | C, E, F, IP, WB, |
| Background | The 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. |
| Concentration | 0.5 mg/ml |
| General Readings | J Biol Chem. 1995 Oct 6;270(40):23681-7. Identification of the 14.3.3 zeta domains important for self-association and Raf binding. Luo ZJ, Zhang XF, Rapp U, Avruch J. Diabetes Research Laboratory, Massachusetts General Hospital, Charlestown 02129, USA. |
| Storage | Store at 4C short term. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Format |
|
| Gene ID | 2810 |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for 14-3-3 protein sigma / SFN (7 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR09318PU-L |
14-3-3 protein sigma / SFN (1-248) |
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Human | Purified | > 95 % | E. coli |
0.5 mg /
€670.00
|
||
| Acris Antibodies GmbH | |||||||
| AR09318PU-N |
14-3-3 protein sigma / SFN (1-248) |
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Human | Purified | > 95 % | E. coli |
0.1 mg /
€250.00
|
||
| Acris Antibodies GmbH | |||||||
| NBP1-30172 |
14-3-3 protein sigma / SFN |
||||||
![]() |
0.1 mg /
€310.00
|
||||||
| Novus Biologicals Inc. | |||||||
| H00002810-P01-10 |
14-3-3 protein sigma / SFN |
||||||
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Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00002810-P01-25 |
14-3-3 protein sigma / SFN |
||||||
![]() |
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00002810-Q01-10 |
14-3-3 protein sigma / SFN |
||||||
![]() |
Human | Purified |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00002810-Q01-25 |
14-3-3 protein sigma / SFN |
||||||
![]() |
Human | Purified |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for 14-3-3 protein sigma / SFN (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-15882 |
14-3-3 sigma Lysate |
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0.1 mg /
€370.00
|
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for 14-3-3 protein sigma / SFN (8 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | Texas Red | |||||
| Acris Antibodies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Rabbit | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Goat | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||


![Wetern Blot: 14-3-3 sigma/Stratifin Protein [NBP1-30172] - 15% SDS-PAGE (3ug)](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/108740_1.jpg)

![Western Blot: 14-3-3 sigma/Stratifin Lysate [NBL1-15882] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for SFN](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/14-3-3 sigma Lysate-Western Blot-NBL1-15882-img0001.jpg)




