AM09060PU-N 14-3-3 protein theta antibody

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0.1 ml / €310.00
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Mouse anti Human 14-3-3 protein theta AT1A1

AM09060PU-N

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Product Description for 14-3-3 protein theta

Mouse anti Human 14-3-3 protein theta AT1A1.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for 14-3-3 protein theta

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ
Presentation Purified
Reactivity Hu
Applications E, P, WB
Clonality Monoclonal
Clone AT1A1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P27348
Immunogen:
Recombinant human 14-3-3 tau (aa 1-245) purified from E. coli
Genename:
10971
Antibody type Ab
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA.
Western blot (1/1,000-1/2,000).
Immunohistochemistry on Paraffin Sections (10 µg/ml).
This YWHAQ antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Background The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.
Concentration 1.0 mg/ml
General Readings
  1. Liu YC, et al., (1996) J Biol Chem 271:14591-14595 Xiao B, et al., (1995) Nature 376:188-191
Storage Store the antibody undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein-G affinity chromatography
Buffer System:
PBS, pH 7.4, containing 0.09% Sodium Azide
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
The antibody recognizes Human 14-3-3 protein theta (tau).
Other species not tested.
Species:
Human
Gene ID 10971
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for 14-3-3 protein theta (5 products)

Catalog No. Species Pres. Purity   Source  
AR09103PU-L

14-3-3 protein theta (1-245)

14-3-3 tau, 1-245 aa: 15% SDS-PAGE (3 µg) Human Purified ≥ 95 by SDS-PAGE E. coli
0.5 mg / €1,000.00
  Acris Antibodies GmbH
AR09103PU-N

14-3-3 protein theta (1-245)

14-3-3 tau, 1-245 aa: 15% SDS-PAGE (3 µg) Human Purified ≥ 95 by SDS-PAGE E. coli
0.1 mg / €390.00
  Acris Antibodies GmbH
NBC1-18393

14-3-3 protein theta

SDS-Page: 14-3-3 theta  Protein [NBC1-18393] - 14-3-3 tau, 27 kDa (245 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE. Protein
0.1 mg / €390.00
  Novus Biologicals Inc.
H00010971-P01-10

14-3-3 protein theta

14-3-3 protein theta Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00010971-P01-25

14-3-3 protein theta

14-3-3 protein theta Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for 14-3-3 protein theta (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-17951

14-3-3 Lysate

Western Blot: 14-3-3 theta  Lysate [NBL1-17951] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for YWHAQ
0.1 mg / €370.00
  Novus Biologicals Inc.
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