Product Description for 14-3-3 protein theta
Mouse anti Human 14-3-3 protein theta AT1A1.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.
Properties for 14-3-3 protein theta
| Product Category | Antibodies |
|---|---|
| Quantity | 50 µl |
| Synonyms | 14-3-3 protein T-cell, 14-3-3 protein tau, FocusOn116, Protein HS1, YWHAQ |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | E, P, WB |
| Clonality | Monoclonal |
| Clone | AT1A1 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Application | ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 µg/ml). This YWHAQ antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. |
| Background | The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis. |
| Concentration | 1.0 mg/ml |
| General Readings |
|
| Storage | Store the antibody undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 10971 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for 14-3-3 protein theta (5 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR09103PU-L |
14-3-3 protein theta (1-245) |
||||||
 |
Human | Purified | ≥ 95 | E. coli |
0.5 mg /
€1,000.00
|
||
| Acris Antibodies GmbH | |||||||
| AR09103PU-N |
14-3-3 protein theta (1-245) |
||||||
|
Human | Purified | ≥ 95 | E. coli |
0.1 mg /
€390.00
|
||
| Acris Antibodies GmbH | |||||||
| NBC1-18393 |
14-3-3 protein theta |
||||||
![SDS-Page: 14-3-3 theta Protein [NBC1-18393] - 14-3-3 tau, 27 kDa (245 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE.](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/14-3-3 Tau Protein-SDS-Page-NBC1-18393-img0001.jpg) |
0.1 mg /
€390.00
|
||||||
| Novus Biologicals Inc. | |||||||
| H00010971-P01-10 |
14-3-3 protein theta |
||||||
 |
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00010971-P01-25 |
14-3-3 protein theta |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for 14-3-3 protein theta (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-17951 |
14-3-3 Lysate |
||||||
![Western Blot: 14-3-3 theta Lysate [NBL1-17951] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for YWHAQ](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/14-3-3 Lysate-Western Blot-NBL1-17951-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for 14-3-3 protein theta (8 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | |||||
| Acris Antibodies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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