14-3-3 protein zeta/delta (222-240) - AM20301PU-N

Product Description for 14-3-3 protein zeta/delta

Mouse anti Bovine, Chicken, Feline, Human, Monkey, Mouse, Porcine, Rat 14-3-3 protein zeta/delta IMG2E11.
Properties: (222-240)
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for 14-3-3 protein zeta/delta

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	FocusOn116, KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ
Presentation	Purified
Reactivity	Bov, Chk, Fe, Hu, Mky, Ms, Por, Rt
Applications	P, WB
Clonality	Monoclonal
Clone	IMG2E11
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P63104 Immunogen: Amino acids 222-240 (RDNLTLWTSDTQGDEAEAG) of Human 14-3-3 zeta. Genename: 7534 Remarks: The amino acid sequence used as immunogen is 100% homologous in Human (100% zeta/delta; 84% eta; 78% alpha/beta; 74% epsilon; 63% gamma), Bovine, Rat, Mouse, Chimpanzee, Rhesus Monkey, Pig, Chicken and Cat, and 94% homologous in Xenopus.
Property	222-240
Antibody type	Ab
Application	Western blot analysis: 1-3 µg/ml. Immunohistochemistry on Paraffin Sections: 5 µg/ml. Recommended Positive Control: Brain.
Background	14-3-3 zeta is a 245 amino acid containing protein belonging to the 14-3-3 family that mediates signal transduction by binding to phosphoserine containing proteins. It is a highly conserved protein in both plants and mammals. The encoded protein interacts with IRS1 implicating a plausible role in regulating insulin sensitivity. There are several transcript variants, with seven known mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta and eta. 14-3-3 proteins function in broad regulation of proteins by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, and activation/repression of enzyme activity. The protein is highly abundant in brain.
Concentration	0.5 mg/ml
General Readings	Murthi, P. et al. Placenta 29:798-801 (2008). Frasor, J. et al. Cancer Res. 66:7334-7340 (2006). Heidenblad, M. et al. BMC Med. Genomics 31:3 (2008).
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch
Format	Purification: Protein G Chromatography Buffer System: PBS containing 0.05% BSA and 0.05% Sodium Azide State: Liquid purified IgG fraction Purified
Specificity	Specificity: Recognizes 14-3-3 zeta Species: Bovine, Cat, Chicken, Chimpanzee, Human, Mouse, Pig, Rat
Gene ID	7534
Targets	14-3-3 protein zeta/delta antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for 14-3-3 protein zeta/delta (6 products)

Catalog No.	Species	Pres.	Purity	Source
AR09104PU-L	14-3-3 protein zeta/delta (1-245, His-tag)
	Human	Purified	≥ 95	E. coli	0.5 mg / €750.00
					Acris Antibodies GmbH
AR09104PU-N	14-3-3 protein zeta/delta (1-245, His-tag)
	Human	Purified	≥ 95	E. coli	0.1 mg / €300.00
					Acris Antibodies GmbH
H00007534-P01-10	14-3-3 protein zeta/delta
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00007534-P01-25	14-3-3 protein zeta/delta
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.
H00007534-Q01-10	14-3-3 protein zeta/delta
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00007534-Q01-25	14-3-3 protein zeta/delta
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for 14-3-3 protein zeta/delta (2 products)

Catalog No.	Species
NBL1-17952	14-3-3 zeta/delta Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.
H00007534-T01	YWHAZ 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for 14-3-3 protein zeta/delta (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH