Product Description for 14-3-3 protein zeta/delta
Rabbit anti Bovine, Chicken, Human, Mouse, Rat, Sheep 14-3-3 protein zeta/delta.
Properties: (N-term acetylated)
Presentation: Serum
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.
Properties: (N-term acetylated)
Presentation: Serum
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.
Properties for 14-3-3 protein zeta/delta
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 ml |
| Synonyms | FocusOn116, KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ |
| Presentation | Serum |
| Reactivity | Bov, Chk, Hu, Ms, Rt, Sh |
| Applications | E, P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
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|---|---|
| Property | N-term acetylated |
| Antibody type | Ab |
| Application | ELISA. Western Blot: 1/3000. Detects a band of approximately 30kDa in HEK293 cell lysates. Immunohistochemistry on Paraffin Sections: 1/400. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Positive Control: Normal brain tissue. |
| Background | It is a member of the 14-3-3 family which consists of 30kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 zeta is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3 zeta also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha. |
| General Readings | 1. Martin, H. et al. (1993) Antibodies against major brain isoforms of 14-3-3 protein. An antibody specific for the Nacetylated aminoterminus of a protein. FEBS 331: 296 - 303. 2. Aitken, A. et al. (2006) 14-3-3 proteins: A historic overview. Seminars in Cancer Biology 16: 162 - 172. 3. Wang, Z. et al. (2011) The prognostic value of 14-3-3 isoforms in vulvar squamous cell carcinoma cases: 14-3-3ß and e are independent prognostic factors for these tumors. PLoS One. 6: e24843. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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| FocusOn and Reviews | |
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Accessory Products
Recommended Secondary Antibodies for 14-3-3 protein zeta/delta (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
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|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
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|
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
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|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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