22941 Tyrosine 3-monooxygenase (TH) antibody

See related secondary antibodies

Search for all "Tyrosine 3-monooxygenase (TH)"

0.1 ml / €410.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti All Species Tyrosine 3-monooxygenase (TH) LNC1

Product Description for Tyrosine 3-monooxygenase (TH)

Mouse anti All Species Tyrosine 3-monooxygenase (TH) LNC1.
Presentation: Ascites
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Tyrosine 3-monooxygenase (TH)

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms TYH, Tyrosine 3-hydroxylase
Presentation Ascites
Reactivity All Species
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone LNC1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer ImmunoStar Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P07101
Immunogen:
TH purified from rat PC12 cells
Genename:
7054
Application IHC Quality Control:
The antibody has wide species cross-reactivity because it recognizes an epitope in the catalytic core of the TH molecule where extensive species homology exists. TH antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat catecholamine neuron systems using indirect immunofluorescent and biotin/avidin-HRP techniques. This antibody does not cross react with phenylalanine hydroxylase or tryptophan hydroxylase.
The immunogen used to produce the TH antibody was full-length TH purified to homogeneity from PC12 cells, which are of rat origin. The exact epitope recognized by this antibody is not known but our studies indicate that following partial digestion of TH with the protease chymotrypsin the antibody recognizes the 34 kDa catalytic core of TH.
HEK293 cells were transiently transfected to express human TH protein. Protein extracts from non-transfected and transfected cells were resolved by SDS-PAGE and transferred to nitrocellulose for western blotting. The blot was probed using our monoclonal antibody to TH and then stripped and re-probed using an antibody to beta-actin. This analysis shows that only extracts derived from cells transfected to express TH contain TH protein identified by the 60 kDa immunoreactive band, while non-transfected and transfected cells both express beta-actin protein.
Format
State:
Lyophilized ascites (100 μL)
Ascites
Specificity
Species:
Amphibian, Chicken, Clam, Crab, Crayfish, Crustacean, Drosophila, Fish, Fly, Frog, Gerbil, Goldfish, Guinea Pig, Hamster, Human, Leech, Lobster, Mollusca, Monkey, Mouse, Newt, Pig, Rabbit, Rat, Snail, Sparrow, Turtle, Worm, Zebra Finch, Zebrafish.
Gene ID 7054

Accessory Products

Proteins and/or Positive Controls

Proteins for Tyrosine 3-monooxygenase (TH) (1 products)

Catalog No. Species Pres. Purity   Source  
NBP1-42461

Tyrosine 3-monooxygenase (TH)

Western Blot: Tyrosine Hydroxylase Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western Blot.  150ng of protein were added to the Western Blot. Human Purified
10 µg / €390.00
  Novus Biologicals Inc.
  • Pinterest
  • LinkedIn