6xHistidine Epitope Tag (HHHHHH) - SM1693P

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag AD1.1.10.
Properties: (HHHHHH)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category	Antibodies
Quantity	1 mg
Presentation	Purified
Applications	C, E, IP, WB
Clonality	Monoclonal
Clone	AD1.1.10
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: PAX6 transcription factor linked to histidine tag. Remarks: Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Antibody type	Ab
Application	ELISA Immunoprecipitation Immunhistology on Frozen Sections. Western blot: 1/500-1/1000. This antibody is routinely tested on Histidine tagged recombinant protein.
Background	The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification.
Concentration	1.0 mg/ml
General Readings	Conrath, K. et al. (2001) Camel single binding-domain antibodies as modular binding units in bispecific and bivalent antibody constructs. J. Biol.Chem. 276 (10): 7346-7350 Suen JL, Wu CH, Chen YY, Wu WM, Chiang BL. Characterization of self-T-cell response and antigenic determinants of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice. Immunology. 2001 Jul;103(3):301-9. PubMed PMID: 11454059. Hoffmann, S.C. et al. (2007) Identification of CLEC12B, an inhibitory receptor on myeloid cells. J. Biol. Chem. 282: 22370-22375. Zheng J, Kohler ME, Chen Q, Weber J, Khan J, Johnson BD, et al. Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen. BMC Immunol. 2007 Mar 30;8:4. PubMed PMID: 17397536. Bahi A, Dreyer JL. Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization. Genes Brain Behav. 2008 Mar;7(2):244-56. Epub 2007 Aug 3. PubMed PMID: 17680802. Wrighton KH, Lin X, Yu PB, Feng XH. Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors. J Biol Chem. 2009 Apr 10;284(15):9755-63. doi: 10.1074/jbc.M809223200. Epub 2009 Feb 18. PubMed PMID: 19224917. Diefenbacher M, Thorsteinsdottir H, Spang A. The Dsl1 tethering complex actively participates in soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex assembly at the endoplasmic reticulum in Saccharomyces cerevisiae. J Biol Chem. 2011 Jul 15;286(28):25027-38. doi: 10.1074/jbc.M110.215657. Epub 2011 Apr 11. PubMed PMID: 21482823. Alvarez MM, López-Pacheco F, Aguilar-Yañez JM, Portillo-Lara R, Mendoza-Ochoa GI, García-Echauri S, et al. Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method. PLoS One. 2010 Apr 14;5(4):e10176. doi: 10.1371/journal.pone.0010176. PubMed PMID: 20418957. Bahi A, Kusnecov A, Dreyer JL. The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement. Neuropsychopharmacology. 2008 Oct;33(11):2726-34. doi: 10.1038/sj.npp.1301668. Epub 2008 Feb 6. PubMed PMID: 18256596. Gunnarsen KS, Lunde E, Kristiansen PE, Bogen B, Sandlie I, Løset GÅ. Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA. BMC Biotechnol. 2010 Feb 3;10:8. doi: 10.1186/1472-6750-10-8. PubMed PMID: 20128915. Hwang HY, Duvall MR, Tomlinson S, Boackle RJ. Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment. Mol Immunol. 2008 May;45(9):2570-80. doi: 10.1016/j.molimm.2007.12.019. Epub 2008 Mar 3. PubMed PMID: 18313756. De Vooght L, Caljon G, Stijlemans B, De Baetselier P, Coosemans M, Van den Abbeele J. Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly. Microb Cell Fact. 2012 Feb 15;11:23. doi: 10.1186/1475-2859-11-23. PubMed PMID: 22335892.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography on Protein G Buffer System: PBS containing 0.09% Sodium Azide State: Liquid purified IgG fraction Purified
Specificity	Specificity: This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags.The antibody may be used to track the purification of proteins expressed in bacteria, Yeast or insect cells.It reacts against all His tagged proteins so far tested.In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or Mammalian cells.

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