6xHistidine Epitope Tag (HHHHHH) - SM1693PS

Product Description for 6xHistidine Epitope Tag

Mouse anti 6xHistidine Epitope Tag AD1.1.10.
Properties: (HHHHHH)
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for 6xHistidine Epitope Tag

Product Category	Antibodies
Quantity	0.1 mg
Presentation	Purified
Applications	C, E, IP, WB
Clonality	Monoclonal
Clone	AD1.1.10
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Immunogen: PAX6 transcription factor linked to Histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Antibody type	Ab
Application	ELISA. Immunoprecipitation. Immunhistology on Frozen Sections. Western blot (1/500-1/1000): This antibody is routinely tested on Histidine tagged recombinant protein.
Background	The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Concentration	1.0 mg/ml
General Readings	Conrath, K. et al. (2001) Camel single binding-domain antibodies as modular binding units in bispecific and bivalent antibody constructs. J. Biol.Chem. 276 (10): 7346-350. Bahi A, Boyer F, Kafri T, Dreyer JL. CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus. Eur J Neurosci. 2004 Mar;19(6):1621-33. PubMed PMID: 15066158.
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity Chromatography on Protein G Buffer System: PBS, pH 7.4, containing 0.09% Sodium Azide as a preservative State: Liquid purified IgG fraction Purified
Specificity	Specificity: This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. We recommend the use of SM1693P for this application.

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