AM32054SU-N ABCC1 / MRP1 antibody

Swiss Prot Num:

P33527

Immunogen:

Bacterial fusion protein of MRP, containing a segment of 170 amino acids in the carboxy terminal end and part of the carboxy proximal nucleotide binding domain of the protein.

Genename:

4363

Mab producing cells: The hybridoma cell line was obtained by fusion of lymph node cells from an immunized mouse (Balb/C) with SP2/0 mouse myeloma cells.
Culture Medium: RPMI-1640 (Gibco, Paisley, Scotland UK), supplemented with Nutridoma-SR (Boehringer, Indianapolis, USA). The medium does not contain serum nor added enzymes. The antibody solution has been filtered through a 0.22 micron filter.
NOTE: This monoclonal antibody has been produced in a clinical laboratory in which no animal viruses are being studied or cultured.

Flow Cytometry: 1/20-1/50. 
Cell permeabilization required with 10% lyzing solution followed by primairy antibody and anti-Mouse-FITC. 
Western blotting: 1/20-1/50.
Immunocytochemistry on Aceton-Fixed Cell Preparation: 1/20-1/50.
Immunohistochemstry on Acetone-Fixed Frozen Sections: 1/20-1/50,
Immunocytochemistry on Formalin-Fixed Paraffin Embedded Tissues 1/20. 
Microwave pretreatment (0.01M Citrate Buffer,10min at 100°C) may increase the performance of this antibody.

• Cole, S., et al., 1992, Science 258, 1650-1654.
• Flens, M., et al., 1994, Cancer Res. 54, 4557-4563.
• Zaman, et al., 1994, Proc.Nat.Acad.Sci. USA 91, 8822-8826.
• Schadendorf, et al, 1995, Am. J. Pathology 147, 1545-52.
• Nooter, et al., 1995, Clin Cancer Res. 1, 1301-10.
• Flens, M., et al., 1996, Am. J. Pathology 148, 1237-47.
• Scheffer et al, Cancer Res. 60:5269-5277, 2000.

Preservatives:

0.09% Sodium Azide

Stabilizers:

0.7% BSA

State:

Serum-Free Tissue Culture Supernatant
Supernatant

Species reactivity (tested):

Human.

Specificity:

This Monoclonal antibody MRPm6 reacts with an internal epitope of MRP, a 180-195 kD transmembrane transporter protein overexpressed in various Human non-P-glycoprotein MDR tumor cell lines. 
MRPm6 does not cross react with the Human MDR1 and MDR3 gene products (Flens et al. 1994).