ABCC11 / MRP8

Product Description for ABCC11 / MRP8

Rat anti Human ABCC11 / MRP8 M8I-74.
Presentation: Supernatant
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Western blot / Immunoblot.

Properties for ABCC11 / MRP8

Product Category	Antibodies
Quantity	1 ml
Synonyms	ATP-binding cassette transporter sub-family C member 11
Presentation	Supernatant
Reactivity	Hu
Applications	C, ICC/IF, WB
Clonality	Monoclonal
Clone	M8I-74
Host	Rat
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: Q96J66 Immunogen: A bacterial fusion protein of Human MRP8, containing amino acids 1-83 of the protein. Genename: 85320
Antibody type	Ab
Application	Western blotting: Use at 1/20-1/50 and anti-Rat-HRP. Immunocytochemistry: Use at 1/20-1/50 on Acetone Fixed Cytospin preparations. Immunohistochemisty on Frozen Sections: 1/20 on Acetone Fixed Frozen sections can be followed by incubation with Rabbit anti-Rat Ig (1/25) and a monoclonal Rat APAAP complex (1/50). Alternatively, after incubation with M8I-74 (use 1/20) and washing, slides can be incubated with biotinylated Rabbit anti-Rat Ig (1/100) and streptavidin conjugated to Horseradish Peroxidase (1/500).
Background	ABCC11 belongs to the ABC transporter family, conjugate transporter (TC 3.A.1.208) subfamily. It contains two ABC transmembrane type-1 domains and two ABC transporter domains. ABCC11 participates in physiological processes involving bile acids, conjugated steroids and cyclic nucleotides and enhances the cellular extrusion of cAMP and cGMP. It also stimulates the ATP-dependent uptake of a range of physiological and synthetic lipophilic anions, including the glutathione S-conjugates leukotriene C4 and dinitrophenyl S-glutathione, steroid sulfates such as dehydroepiandrosterone 3-sulfate (DHEAS) and estrone 3-sulfate, glucuronides such as estradiol 17-beta-D-glucuronide (E(2)17betaG), the monoanionic bile acids glycocholate and taurocholate, and methotrexate. It probably functions to secrete earwax.
Concentration	0.25 mg/ml (by ELISA)
General Readings	ABCC10, ABCC11, and ABCC12. Kruh GD, Guo Y, Hopper-Borge E, Belinsky MG, Chen ZS. Pflugers Arch. 2007 Feb;453(5):675-84 de Wolf CJ, Yamaguchi H, van der Heijden I, Wielinga PR, Hundscheid SL, Ono N, et al. cGMP transport by vesicles from human and mouse erythrocytes. FEBS J. 2007 Jan;274(2):439-50. PubMed PMID: 17229149. MRP8/ABCC11 directly confers resistance to 5-fluorouracil. Oguri T, Bessho Y, Achiwa H, Ozasa H, Maeno K, Maeda H, Sato S, Ueda R. Mol Cancer Ther. 2007 Jan;6(1):122-7 Park S, Shimizu C, Shimoyama T, Takeda M, Ando M, Kohno T, et al. Gene expression profiling of ATP-binding cassette (ABC) transporters as a predictor of the pathologic response to neoadjuvant chemotherapy in breast cancer patients. Breast Cancer Res Treat. 2006 Sep;99(1):9-17. Epub 2006 Jun 5. PubMed PMID: 16752223. Shimoyama T, Takeda M, Ando M, Kohno T, Katsumata N, Kang YK, Nishio K, Fujiwara Y. Breast Cancer Res Treat. 2006 Sep;99(1):9-17. Yoshiura K, Kinoshita A, Ishida T, Ninokata A, Ishikawa T, Kaname T, et al. A SNP in the ABCC11 gene is the determinant of human earwax type. Nat Genet. 2006 Mar;38(3):324-30. Epub 2006 Jan 29. PubMed PMID: 16444273.
Storage	Store antibody undiluted at 2-8°C. Shelf life: one year from despatch.
Format	Preservatives: 0.09% Sodium Azide Stabilizers: 0.7% BSA State: Serum Free Culture Supernatant Supernatant
Species Reactivity	Species reactivity (tested): Human.
Specificity	Specificity: This Monoclonal antibody clone M8I-74 reacts with an internal epitope of MRP8 (ABCC11), an approximately 150 kD transmembrane protein that is related to the multidrug resistance protein MRP1.
Gene ID	85320
Targets	ABCC11 / MRP8 antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for ABCC11 / MRP8 (3 products)

Catalog No.	Species	Pres.
H00085320-P01	ABCC11 / MRP8
	Human	Purified	2 µg / €250.00
			Abnova Taiwan Corp.
H00085320-Q01-10	ABCC11 / MRP8
	Human	Purified	10 µg / €350.00
			Abnova Taiwan Corp.
H00085320-Q01-25	ABCC11 / MRP8
	Human	Purified	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for ABCC11 / MRP8 (1 products)

Catalog No.	Species
H00085320-T01	ABCC11 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €240.00
		Abnova Taiwan Corp.

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