AM10020PU-N ACTA2 / aortic smooth muscle Actin antibody

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1.0 ml / €250.00
Please visit the appropriate website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Rabbit, Rat ACTA2 / aortic smooth muscle Actin Aasm 204 (1A4)

AM10020PU-N

Product Description for ACTA2 / aortic smooth muscle Actin

Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Rabbit, Rat ACTA2 / aortic smooth muscle Actin Aasm 204 (1A4).
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for ACTA2 / aortic smooth muscle Actin

Product Category Antibodies
Quantity 1.0 ml
Synonyms ACTSA, ACTVS, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Presentation Purified
Reactivity Bov, Chk, Hu, Mky, Ms, Rb, Rt
Applications C, ICC/IF, P
Clonality Monoclonal
Clone Aasm 204 (1A4)
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P62736
Immunogen:
N-terminal decapeptide of alpha smooth muscle isoform of actin, acetylated at the N-terminus.
Genename:
59
Remarks:
Epitope: Acetyl group with N- terminal four amino acids of peptide chain of alpha smooth muscle.
Antibody type Ab
Application Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears)
For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature.
Recommended Positive Control: Human uterus, gastrointestinal tissue, Leiomyosarcoma.
Background Actin is a globular, roughly 42-kDa protein found in all eukaryotic cells. Actin participates in many important cellular functions, including muscle contraction cell motility cell division and cytokinesis vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Expression of smooth muscle alpha actin is regulated by hormones and cell proliferation and is altered by pathological conditions including onogenic transformation and atherosclerosis.
Concentration 0.2 mg/ml
General Readings
  • Skalli, O et. al. J of cell Biology, 103: 2787-2796, 1986.
  • Miwa, T et. al. Mol. cell Biology, 11: 3296-3304, 1991
Storage Store the antibody undiluted at 2-8 °C.
Shelf life: One year from despatch.
Format
Buffer System:
PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative.
State:
Liquid purified Ig fraction.
Purified
Specificity
Specificity:
This antibody recognises ACTIN alpha Smooth Muscle.
Cellular Localization: Cytoplasmic.
Species:
Human, Baboon, Bovine, Rabbit, Rat, Mouse and Chicken.
Gene ID 59
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for ACTA2 / aortic smooth muscle Actin (2 products)

Catalog No. Species Pres. Purity   Source  
H00000059-P01-10

ACTA2 / aortic smooth muscle Actin

ACTA2 / aortic smooth muscle Actin Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00000059-P01-25

ACTA2 / aortic smooth muscle Actin

ACTA2 / aortic smooth muscle Actin Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for ACTA2 / aortic smooth muscle Actin (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-07272

Actin Alpha 2 Smooth Muscle Lysate

Western Blot: Actin Alpha 2 Smooth Muscle Lysate [NBL1-07272] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for ACTA2
0.1 mg / €370.00
  Novus Biologicals Inc.