ACTA2 / aortic smooth muscle Actin

Product Description for ACTA2 / aortic smooth muscle Actin

Mouse anti Bovine, Chicken, Human, Mouse, Rabbit, Rat ACTA2 / aortic smooth muscle Actin 1A4.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for ACTA2 / aortic smooth muscle Actin

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	ACTSA, ACTVS, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Presentation	Purified
Reactivity	Bov, Chk, Hu, Ms, Rb, Rt
Applications	ICC/IF, P
Clonality	Monoclonal
Clone	1A4
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P62736 Immunogen: A synthetic peptide from N-terminus of Human alpha smooth muscle isoform of Actin. Epitope: N-terminus Genename: 59
Antibody type	Ab
Add. information	Molecular Weight: 42 kDa
Application	Immunofluorescence. Immunohistochemistry with Formalin-Fixed, Paraffin-Embedded tissues: 1/200. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Incubation Time: 30 minutes at RT. Positive Control: Smooth Muscle or leimyosarcoma.
Background	Actin is a major component of the cytoskeleton and is present in every cell type. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta and gamma in order of increasing isoelectric point. Smooth Muscle Actin Ab does not stain cardiac or skeletal muscle. However, it will stain myofibroblasts and myoepithelial cells. This antibody could be used together with Muscle Specific Actin to distinguish leiomyosarcoma from rhabdomyosarcoma.
Storage	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
Format	Purification: Protein A/G chromatography Buffer System: 10mM PBS, pH 7.4 with 1% BSA as stabilizer and 0.09% Sodium Azide as preservative State: Liquid purified Ig fraction Purified
Species Reactivity	Species reactivity (expected): Baboon, Bovine, Rabbit, Mouse, Rat. Species reactivity (tested): Human.
Specificity	Specificity: This antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells. Actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), and myocardium (alpha-myocardial) are not labeled. Cellular Localization: Cytoplasm.
Gene ID	59
FocusOn and Reviews	Reviews: Antibody Panel to Actin - Product Review 08
Targets	ACTA2 / aortic smooth muscle Actin antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for ACTA2 / aortic smooth muscle Actin (2 products)

Catalog No.	Species	Source
H00000059-P01-10	ACTA2 / aortic smooth muscle Actin
	Human	in vitro transl.	10 µg / €350.00
			Abnova Taiwan Corp.
H00000059-P01-25	ACTA2 / aortic smooth muscle Actin
	Human	in vitro transl.	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for ACTA2 / aortic smooth muscle Actin (1 products)

Catalog No.	Species
NBL1-07272	Actin Alpha 2 Smooth Muscle Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for ACTA2 / aortic smooth muscle Actin (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH