ACTA2 / aortic smooth muscle Actin

Product Description for ACTA2 / aortic smooth muscle Actin

Mouse anti Bovine, Chicken, Human, Mouse, Rabbit, Rat ACTA2 / aortic smooth muscle Actin 1A4/asm-1.
Presentation: Purified
Product is tested for Paraffin Sections.

Properties for ACTA2 / aortic smooth muscle Actin

Product Category	Antibodies
Quantity	1 ml
Synonyms	ACTSA, ACTVS, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Presentation	Purified
Reactivity	Bov, Chk, Hu, Ms, Rb, Rt
Applications	P
Clonality	Monoclonal
Clone	1A4/asm-1
Host	Mouse
Isotype	IgG2a
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P62736 Immunogen: N-terminal decapeptide of alpha-smooth muscle Actin Genename: 59
Antibody type	Ab
Application	Immunohistochemistry on Formalin Fixed Paraffin Embedded Sections: Use Actin antibody at 1/25-1/50 dilution in an ABC method for 30 minutes at room temperature. Recommended Positive Control: Leiomyoma.
Background	Smooth muscle actin is expressed in normal smooth muscle and in myoepithelial cells, and also in tumours derived from these cells. Actins are involved in maintaining the structure and integrity of a cell, as well as cell motility. They are highly conserved proteins and make up the majority of the contractile apparatus. To date, six different actin isoforms have been described.
General Readings	Skalli et al. Am J Cell Biol 103: 2787, 1986. Skalli et al. Lab Invest 60: 275, 1989. Skalli et al. J Histo Chem 37: 315, 1989. O'Byrne et al. Br J cancer 82: 1427, 2000.
Storage	Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.
Format	State: Liquid purified Ig fraction containing Sodium Azide as preservative Purified
Specificity	Specificity: This antibody is specific to alpha-smooth isoform of actin. It reacts with smooth muscle cells of vessels and different parenchymes. The antibody does not cross-react with beta and gamma-cytoplasmic, alpha-sarcomeric and alpha-myocardial actin isoforms. Cellular Localization: Cytoplasmic. Species: Bovine, Chicken, Human, Mouse, Rabbit and Rat.
Gene ID	59
FocusOn and Reviews	Reviews: Antibody Panel to Actin - Product Review 08
Targets	ACTA2 / aortic smooth muscle Actin antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for ACTA2 / aortic smooth muscle Actin (2 products)

Catalog No.	Species	Source
H00000059-P01-10	ACTA2 / aortic smooth muscle Actin
	Human	in vitro transl.	10 µg / €350.00
			Abnova Taiwan Corp.
H00000059-P01-25	ACTA2 / aortic smooth muscle Actin
	Human	in vitro transl.	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for ACTA2 / aortic smooth muscle Actin (1 products)

Catalog No.	Species
NBL1-07272	Actin Alpha 2 Smooth Muscle Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for ACTA2 / aortic smooth muscle Actin (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH