Product Description for Actin beta / ACTB
Mouse anti Bovine, Canine, Chicken, Feline, Fish, Guinea Pig, Human, Leech, Mouse, Porcine, Rabbit, Rat, Sheep Actin beta / ACTB AC-15.
Presentation: Ascites
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.
Presentation: Ascites
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.
Properties for Actin beta / ACTB
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 ml |
| Synonyms | Actin cytoplasmic 1, Beta-Actin, FocusOn117, Review 008 |
| Presentation | Ascites |
| Reactivity | Bov, Can, Chk, Fe, Fi, GP, Hu, Le, Ms, Por, Rb, Rt, Sh |
| Applications | C, E, ICC/IF, P, WB |
| Clonality | Monoclonal |
| Clone | AC-15 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Application | ELISA, Immunocytochemistry, immunohistochemistry, immunohistochemistry, Western Blot, This antibody is useful for Western blot, ELISA, immunohistochemistry and Immunocytochemistry. Molecular weight of beta actin is 42kD. The epitope recognized by the antibody is resistant to formalin fixation, acetone fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives)., ELISA , immunohistochemistry , immunohistochemistry , Western Blot 1:5000, Immunocytochemistry 1:1000, |
| Background | Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. |
| Concentration | 2.8 mg/ml |
| General Readings |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Format |
|
| Specificity |
|
| Gene ID | 60 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for Actin beta / ACTB (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00000060-P01-10 |
Actin beta / ACTB |
||||||
 |
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000060-P01-25 |
Actin beta / ACTB |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Actin beta / ACTB (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| L015T6 |
Actin beta / ACTB Lysate (Non-Denatured) |
||||||
|
0.1 mg /
€240.00
|
||||||
| Abnova Taiwan Corp. | |||||||
Recommended Secondary Antibodies for Actin beta / ACTB (8 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | |||||
| Acris Antibodies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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