Actin beta / ACTB

Product Description for Actin beta / ACTB

Mouse anti Bovine, Canine, Chicken, Drosophila, Feline, Fish, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep Actin beta / ACTB AC-15.
Presentation: DyLight488
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for Actin beta / ACTB

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	Actin cytoplasmic 1, Beta-Actin, FocusOn117, Review 008
Presentation	DyLight488
Reactivity	Bov, Can, Chk, Dros, Fe, Fi, GP, Hu, Ms, Por, Rb, Rt, Sh
Applications	E, ICC/IF, P, WB
Clonality	Monoclonal
Clone	AC-15
Host	Mouse
Isotype	IgG1
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P60709 Immunogen: Synthetic peptide conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). Genename: 60
Antibody type	Ab
Application	ELISA, Immunocytochemistry, immunofluorescence, immunohistochemistry, Western Blot, This is an ideal reagent for use as a cell extract loading control on Western blot. It has been used as such in most of the references below. Molecular weight of beta actin is 42kD. The epitope recognized by the antibody is resistant to formalin fixation, acetone fixation and paraffin embedding. B5, methacarn, ethanol or Bouin's solutions may also be used as fixatives)., ELISA , immunohistochemistry , immunofluorescence , Western Blot 1:5000, Immunocytochemistry 1:1000,
Background	Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Concentration	1 mg/ml
Storage	Store at 4C. Do not freeze.
Format	Label: DyLight 488 DyLight488 Purification: Ascites Purity: Ascites
Specificity	Specificity: N-terminus of the beta isoform of actin., Beta-actin (AC-15) Species: Bovine, Canine, Chicken, Drosophila, Feline, Fish, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
Gene ID	60
FocusOn and Reviews	Reviews: Antibody Panel to Actin - Product Review 08
Targets	Actin beta / ACTB antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Actin beta / ACTB (2 products)

Catalog No.	Species	Source
H00000060-P01-10	Actin beta / ACTB
	Human	in vitro transl.	10 µg / €350.00
			Abnova Taiwan Corp.
H00000060-P01-25	Actin beta / ACTB
	Human	in vitro transl.	25 µg / €500.00
			Abnova Taiwan Corp.

Positive controls for Actin beta / ACTB (1 products)

Catalog No.	Species
L015T6	Actin beta / ACTB Lysate (Non-Denatured)
		0.1 mg / €240.00
		Abnova Taiwan Corp.

Recommended Secondary Antibodies for Actin beta / ACTB (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH