Actin beta / ACTB - NB600-503 | acris-antibodies.com

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Product Description for Actin beta / ACTB

Rabbit anti Human, Monkey, Mouse, Rat Actin beta / ACTB.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for Actin beta / ACTB

Product Category	Antibodies
Quantity	50 µl
Synonyms	Actin cytoplasmic 1, Beta-Actin, FocusOn117, Review 008
Presentation	Aff - Purified
Reactivity	Hu, Mky, Ms, Rt
Applications	IP, P, WB
Clonality	Polyclonal
Host	Rabbit
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P60709 Immunogen: Synthetic peptide derived from the N-terminus of Human Beta-Actin. Genename: 60
Antibody type	Ab
Application	Immunocytochemistry, immunofluorescence, immunoprecipitation, Western Blot, This antibody is useful for Western blotting, Immunoprecipitation, Immunofluorescence and Immunocytochemistry, Immunocytochemistry, immunofluorescence, immunoprecipitation, Western Blot
Background	Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Concentration	1 mg/ml
General Readings	Boutz PL, Chawla G, Stoilov P, Black DL. MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development. Genes Dev. 2007 Jan 1;21(1):71-84. PubMed PMID: 17210790. Brault S, Gobeil F, Fortier A, Honoré JC, Joyal JS, Sapieha PS, et al. Lysophosphatidic acid induces endothelial cell death by modulating the redox environment. Am J Physiol Regul Integr Comp Physiol. 2007 Mar;292(3):R1174-83. Epub 2006 Nov 22. PubMed PMID: 17122328. Cambonie, G., Comte, B., Yzydorczyk, C., Ntimbane, T., Germain, N., Le, N. L. O., Pladys, P., Gauthier, C., Lahaie, I., Abran, D., Lavoie, J.-C., and Nuyt, A. M. (2007) Antenatal antioxidant prevents adult hypertension, vascular dysfunction, and microvascular rarefaction associated with in utero exposure to a low-protein diet, 292, R1236-1245. Carlson D, Maass DL, White DJ, Tan J, Horton JW. Antioxidant vitamin therapy alters sepsis-related apoptotic myocardial activity and inflammatory responses. Am J Physiol Heart Circ Physiol. 2006 Dec;291(6):H2779-89. Epub 2006 Jul 14. PubMed PMID: 16844910. Csiszar A, Ahmad M, Smith KE, Labinskyy N, Gao Q, Kaley G, et al. Bone morphogenetic protein-2 induces proinflammatory endothelial phenotype. Am J Pathol. 2006 Feb;168(2):629-38. PubMed PMID: 16436676. Dennis VA, Jefferson A, Singh SR, Ganapamo F, Philipp MT. Interleukin-10 anti-inflammatory response to Borrelia burgdorferi, the agent of Lyme disease: a possible role for suppressors of cytokine signaling 1 and 3. Infect Immun. 2006 Oct;74(10):5780-9. PubMed PMID: 16988256. Garg A, Barnes PF, Porgador A, Roy S, Wu S, Nanda JS, et al. Vimentin expressed on Mycobacterium tuberculosis-infected human monocytes is involved in binding to the NKp46 receptor. J Immunol. 2006 Nov 1;177(9):6192-8. PubMed PMID: 17056548. Johnson JD, Bernal-Mizrachi E, Alejandro EU, Han Z, Kalynyak TB, Li H, et al. Insulin protects islets from apoptosis via Pdx1 and specific changes in the human islet proteome. Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19575-80. Epub 2006 Dec 8. PubMed PMID: 17158802. Jović M, Naslavsky N, Rapaport D, Horowitz M, Caplan S. EHD1 regulates beta1 integrin endosomal transport: effects on focal adhesions, cell spreading and migration. J Cell Sci. 2007 Mar 1;120(Pt 5):802-14. Epub 2007 Feb 6. PubMed PMID: 17284518. Koivunen P, Hirsilä M, Remes AM, Hassinen IE, Kivirikko KI, Myllyharju J. Inhibition of hypoxia-inducible factor (HIF) hydroxylases by citric acid cycle intermediates: possible links between cell metabolism and stabilization of HIF. J Biol Chem. 2007 Feb 16;282(7):4524-32. Epub 2006 Dec 19. PubMed PMID: 17182618. Nadauld LD, Phelps R, Moore BC, Eisinger A, Sandoval IT, Chidester S, et al. Adenomatous polyposis coli control of C-terminal binding protein-1 stability regulates expression of intestinal retinol dehydrogenases. J Biol Chem. 2006 Dec 8;281(49):37828-35. Epub 2006 Oct 6. PubMed PMID: 17028196. Oganesian A, Au S, Horst JA, Holzhausen LC, Macy AJ, Pace JM, et al. The NH2-terminal propeptide of type I procollagen acts intracellularly to modulate cell function. J Biol Chem. 2006 Dec 15;281(50):38507-18. Epub 2006 Oct 3. PubMed PMID: 17018525. Ostroukhova M, Qi Z, Oriss TB, Dixon-McCarthy B, Ray P, Ray A. Treg-mediated immunosuppression involves activation of the Notch-HES1 axis by membrane-bound TGF-beta. J Clin Invest. 2006 Apr;116(4):996-1004. Epub 2006 Mar 16. PubMed PMID: 16543950. Park KH, Hallows JL, Chakrabarty P, Davies P, Vincent I. Conditional neuronal simian virus 40 T antigen expression induces Alzheimer-like tau and amyloid pathology in mice. J Neurosci. 2007 Mar 14;27(11):2969-78. PubMed PMID: 17360920. Sirinyan M, Sennlaub F, Dorfman A, Sapieha P, Gobeil F, Hardy P, et al. Hyperoxic exposure leads to nitrative stress and ensuing microvascular degeneration and diminished brain mass and function in the immature subject. Stroke. 2006 Nov;37(11):2807-15. Epub 2006 Sep 28. PubMed PMID: 17008616. Wang W, Hayashi J, Serrero G. PC cell-derived growth factor confers resistance to dexamethasone and promotes tumorigenesis in human multiple myeloma. Clin Cancer Res. 2006 Jan 1;12(1):49-56. PubMed PMID: 16397023. Younis I, Boris-Lawrie K, Green PL. Human T-cell leukemia virus open reading frame II encodes a posttranscriptional repressor that is recruited at the level of transcription. J Virol. 2006 Jan;80(1):181-91. PubMed PMID: 16352542. Sané AT, Sinnett D, Delvin E, Bendayan M, Marcil V, Ménard D, et al. Localization and role of NPC1L1 in cholesterol absorption in human intestine. J Lipid Res. 2006 Oct;47(10):2112-20. Epub 2006 Jul 7. PubMed PMID: 16829661.
Storage	Store at 4C. Do not freeze.
Format	Purification: Immunogen affinity purified Buffer System: 0.09% Sodium Azide Purity: Immunogen affinity purified State: Aff - Purified
Specificity	Specificity: Beta actin Species: Human, Mouse, Primate, Rat
Gene ID	60
FocusOn and Reviews	Reviews: Antibody Panel to Actin - Product Review 08
Targets	Actin beta / ACTB antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Actin beta / ACTB (2 products)

Catalog No.	Species	Pres.	Purity		Source
H00000060-P01-10	Actin beta / ACTB
	Human				in vitro transl.		10 µg / €350.00
							Abnova Taiwan Corp.
H00000060-P01-25	Actin beta / ACTB
	Human				in vitro transl.		25 µg / €500.00
							Abnova Taiwan Corp.

Positive controls for Actin beta / ACTB (1 products)

Catalog No.	Species	Pres.	Purity		Source
L015T6	Actin beta / ACTB Lysate (Non-Denatured)
							0.1 mg / €240.00
							Abnova Taiwan Corp.

Recommended Secondary Antibodies for Actin beta / ACTB (6 products)

Catalog No.	Host	Clone/Iso.	Pres.		React.	Applications
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat		AP
							Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat		Biotin
							Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat		FITC
							Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat		HRP
							Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat		TRITC
							Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey		PE
							Acris Antibodies GmbH

Click here to see all secondary antibodies for Actin beta / ACTB

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