Product Description for AKT1 / PKB
Mouse anti Canine, Human, Mouse, Rat AKT1 / PKB 11A11.
Properties: non pSer473 (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties: non pSer473 (incl. pos. control)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties for AKT1 / PKB
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, Review 010 |
| Presentation | Purified |
| Reactivity | Can, Hu, Ms, Rt |
| Applications | E, WB |
| Clonality | Monoclonal |
| Clone | 11A11 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | non pSer473 |
| Antibody type | Ab |
| Add. information | A positive control cell lysate is provided - please see "Protocols" for details. |
| Application | Suitable for Western blot: 0.5 µg/ml for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated HepG2 cells (provided with this antibody). ELISA: 0.05 µg/ml. |
| Background | The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway. |
| Protocols | POSITIVE CONTROL CELL LYSATE: HepG2 untreated Format: Lyophilized cell lysate from serum starved HepG2 cells Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen, avoid repeated freezing and thawing. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. PLEASE NOTE: The lyophilized cell lysates contain SDS and are NOT recommended for applications with native proteins such as immunoprecipitation. |
| Storage | For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. Avoid repeated freeze / thaw cycles. |
| Format |
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| Specificity |
|
| Gene ID | 207 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for AKT1 / PKB (9 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR08011PU-N |
AKT1 / PKB (Substrate) |
||||||
 |
Human | Purified | > 98 % |
0.5 mg /
€220.00
|
|||
| Acris Antibodies GmbH | |||||||
| GTX29041 |
AKT1 / PKB control peptide |
||||||
|
50 µg /
€250.00
|
||||||
| GeneTex Inc. | |||||||
| H00000207-P01-10 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-P01-25 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q01-10 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q01-25 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02 |
AKT1 / PKB |
||||||
|
Human | Purified |
10 µg /
€330.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02-10 |
AKT1 / PKB |
||||||
|
Human | Purified |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02-25 |
AKT1 / PKB |
||||||
|
Human | Purified |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for AKT1 / PKB (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-07441 |
AKT1 Lysate |
||||||
![Western Blot: AKT1 Lysate [NBL1-07441] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for AKT1](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/AKT1 Lysate-Western Blot-NBL1-07441-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for AKT1 / PKB (8 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | |||||
| Acris Antibodies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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