AKT1 / PKB

Product Description for AKT1 / PKB

Rabbit anti Human, Mouse, Rat AKT1 / PKB.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for AKT1 / PKB

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, Review 010
Presentation	Aff - Purified
Reactivity	Hu, Ms, Rt
Applications	E, ICC/IF, P, WB
Clonality	Polyclonal
Host	Rabbit
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P31749 Genename: 207
Antibody type	Ab
Application	ELISA: 1:1000~1:10000. WB: 1:500~1:1000. IHC: 1:50~1:200. IF: 1:50~1:200.
Background	The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-I (IGF-I) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-I-stimulated cells by an upstream kinase(s).
Concentration	1,0 mg/ml
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Affinity chromatography Buffer System: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. State: Liquid purified Ig Aff - Purified
Specificity	Specificity: Akt antibody detects endogenous levels of Akt protein. Species: Human, Mouse, Rat.
Gene ID	207
FocusOn and Reviews	Reviews: Antibody Panel to AKT Protein Family - Product Review 10
Targets	AKT1 / PKB antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for AKT1 / PKB (9 products)

Catalog No.	Species	Pres.	Purity	Source
AR08011PU-N	AKT1 / PKB (Substrate)
	Human	Purified	> 98 %		0.5 mg / €220.00
					Acris Antibodies GmbH
GTX29041	AKT1 / PKB control peptide
					50 µg / €250.00
					GeneTex Inc.
H00000207-P01-10	AKT1 / PKB
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-P01-25	AKT1 / PKB
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00000207-Q01-10	AKT1 / PKB
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-Q01-25	AKT1 / PKB
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00000207-Q02	AKT1 / PKB
	Human	Purified			10 µg / €330.00
					Abnova Taiwan Corp.
H00000207-Q02-10	AKT1 / PKB
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-Q02-25	AKT1 / PKB
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for AKT1 / PKB (1 products)

Catalog No.	Species
NBL1-07441	AKT1 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for AKT1 / PKB (6 products)

Catalog No.	Host	Pres.
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat	AP
			Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat	Biotin
			Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat	FITC
			Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat	HRP
			Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat	TRITC
			Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey	PE
			Acris Antibodies GmbH