Product Description for AKT1 / PKB
Rabbit anti Human, Mouse, Rat AKT1 / PKB.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.
Properties for AKT1 / PKB
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, Review 010 |
| Presentation | Aff - Purified |
| Reactivity | Hu, Ms, Rt |
| Applications | E, P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Application | ELISA: 1:5000~1:10000. WB: 1:500~1:1000. IHC: 1:50~1:200. |
| Background | AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. |
| Concentration | 1,0 mg/ml |
| Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
| Format |
|
| Specificity |
|
| Gene ID | 207 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for AKT1 / PKB (9 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR08011PU-N |
AKT1 / PKB (Substrate) |
||||||
 |
Human | Purified | > 98 % |
0.5 mg /
€220.00
|
|||
| Acris Antibodies GmbH | |||||||
| GTX29041 |
AKT1 / PKB control peptide |
||||||
|
50 µg /
€250.00
|
||||||
| GeneTex Inc. | |||||||
| H00000207-P01-10 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-P01-25 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q01-10 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q01-25 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02 |
AKT1 / PKB |
||||||
|
Human | Purified |
10 µg /
€330.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02-10 |
AKT1 / PKB |
||||||
|
Human | Purified |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02-25 |
AKT1 / PKB |
||||||
|
Human | Purified |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for AKT1 / PKB (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-07441 |
AKT1 Lysate |
||||||
![Western Blot: AKT1 Lysate [NBL1-07441] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for AKT1](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/AKT1 Lysate-Western Blot-NBL1-07441-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for AKT1 / PKB (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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