Product Description for AKT1 / PKB
Rabbit anti Human AKT1 / PKB.
Properties: (N-term)
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Western blot / Immunoblot.
Properties: (N-term)
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Western blot / Immunoblot.
Properties for AKT1 / PKB
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, Review 010 |
| Reactivity | Hu |
| Applications | E, F, ICC/IF, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | Acris Antibodies GmbH |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | N-term |
| Antibody type | Ab |
| Control peptide | AP17878CP-N |
| Application | ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunofluorescence: 1/10-1/50. Flow Cytometry: 1/10-1/50. |
| Background | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. |
| Concentration | 0.25 mg/ml |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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| Gene ID | 207 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for AKT1 / PKB (9 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR08011PU-N |
AKT1 / PKB (Substrate) |
||||||
 |
Human | Purified | > 98 % |
0.5 mg /
€220.00
|
|||
| Acris Antibodies GmbH | |||||||
| GTX29041 |
AKT1 / PKB control peptide |
||||||
|
50 µg /
€250.00
|
||||||
| GeneTex Inc. | |||||||
| H00000207-P01-10 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-P01-25 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q01-10 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q01-25 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02 |
AKT1 / PKB |
||||||
|
Human | Purified |
10 µg /
€330.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02-10 |
AKT1 / PKB |
||||||
|
Human | Purified |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02-25 |
AKT1 / PKB |
||||||
|
Human | Purified |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for AKT1 / PKB (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-07441 |
AKT1 Lysate |
||||||
![Western Blot: AKT1 Lysate [NBL1-07441] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for AKT1](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/AKT1 Lysate-Western Blot-NBL1-07441-img0001.jpg) |
0.1 mg /
€370.00
|
||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for AKT1 / PKB (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
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