Product Description for AKT1 / PKB
Chicken anti Bovine, Human, Mouse, Rat, Xenopus AKT1 / PKB.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.
Properties for AKT1 / PKB
| Product Category | Antibodies |
|---|---|
| Quantity | 50 µg |
| Synonyms | Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, Review 010 |
| Presentation | Aff - Purified |
| Reactivity | Bov, Hu, Ms, Rt, Xen |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Chicken |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Add. information | Research Areas: Cancer, Neuroscience |
| Application | immunohistochemistry, Western Blot, WB: Predicted molecular weight: 58 kDa. Optimal dilutions/concentrations should be determined by the end user., immunohistochemistry 1/200, Western Blot 1/500 - 1/2000 |
| Background | The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene). |
| Concentration | 1 mg/ml |
| General Readings | General / background references:Brunet A et al. Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Cell 96:857-68 (1999). |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Format |
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| Specificity |
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| Gene ID | 207 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for AKT1 / PKB (9 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| AR08011PU-N |
AKT1 / PKB (Substrate) |
||||||
 |
Human | Purified | > 98 % |
0.5 mg /
€220.00
|
|||
| Acris Antibodies GmbH | |||||||
| GTX29041 |
AKT1 / PKB control peptide |
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|
50 µg /
€250.00
|
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| GeneTex Inc. | |||||||
| H00000207-P01-10 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-P01-25 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q01-10 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q01-25 |
AKT1 / PKB |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02 |
AKT1 / PKB |
||||||
|
Human | Purified |
10 µg /
€330.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02-10 |
AKT1 / PKB |
||||||
|
Human | Purified |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000207-Q02-25 |
AKT1 / PKB |
||||||
|
Human | Purified |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for AKT1 / PKB (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-07441 |
AKT1 Lysate |
||||||
![Western Blot: AKT1 Lysate [NBL1-07441] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for AKT1](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/AKT1 Lysate-Western Blot-NBL1-07441-img0001.jpg) |
0.1 mg /
€370.00
|
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for AKT1 / PKB (5 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1300AP |
Chicken IgG / Chicken IgY (H+L chain) antibody |
||||||
|
|
Rabbit | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1300B |
Chicken IgG / Chicken IgY (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1300F |
Chicken IgG / Chicken IgY (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1300HRP |
Chicken IgG / Chicken IgY (H+L chain) antibody |
||||||
|
|
Rabbit | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1300T |
Chicken IgG / Chicken IgY (H+L chain) antibody |
||||||
|
|
Rabbit | TRITC | |||||
| Acris Antibodies GmbH | |||||||
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