AKT1 / PKB

Product Description for AKT1 / PKB

Rabbit anti Human, Mouse AKT1 / PKB.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for AKT1 / PKB

Product Category	Antibodies
Quantity	0.1 ml
Synonyms	Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, Review 010
Presentation	Aff - Purified
Reactivity	Hu, Ms
Applications	WB
Clonality	Polyclonal
Host	Rabbit
Isotype	IgG
Shipping to	Not USA/Canada
PDF datasheet	View Datasheet
Manufacturer	Novus Biologicals Inc.

Datasheet Extract

Immunogen	Swiss Prot Num: P31749 Immunogen: Synthetic peptide derived from a region of AKT that contains threonine 308. Genename: 207
Antibody type	Ab
Add. information	Research Areas: Cancer, Neuroscience
Application	Western Blot, WB: Use at a concentration of 1:1000. Predicted molecular weight: 65 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., Western Blot
Background	Phosphorylation site specific antibody selective for the phosphorylated form of the AKT protein containing a phosphate on threonine 308. The antibody has been shown to recognize AKT in NIH-3T3 cells stimulated with PDGF. AKT is a key effector of the PI3K pathway. Several studies have shown that a signalling pathway from PI3K to AKT may mediate some cellular responses of PI3K, including protection from apoptosis in PC12 cells. AKT accomplishes its cell survival-promoting function by inhibiting apoptosis by means of its ability to phosphorylate and inactivate BAD and caspase 9. The function of AKT is regulated by phosphorylation on two sites conserved within the AGC kinase family: the activation loop (threonine 308) in the kinase core and hydrophobic phosphorylation site on the carboxyl terminus (serine 473). Threonine 308 is phosphorylated by the phosphoinositide-dependent kinase 1 (PDK 1).
Concentration	0.5 mg/ml
General Readings	Franke TF et al. PI3K: downstream AKTion blocks apoptosis. Cell 88:435-7 (1997). Carpenter CL & Cantley LC Phosphoinositide kinases. Curr Opin Cell Biol 8:153-8 (1996). Burgering BM & Coffer PJ Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376:599-602 (1995). Franke TF et al. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81:727-36 (1995). 5. Beffert, U., et al. (2002). Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta. JBC. 277(51): 49958-49964. 6. Hsu, J., et al. (2001). The AKT kinase is activated in multiple myeloma tumor cells. Blood 98(9): 2853-2855. 7. Persad, S., et al. (2001). Regulation of protein kianse B/Akt-theronine-308 phosphorylation by integrin kinked kinase (ilk): Critical roles for kinase activity and amino acids arginine-211 and threonine-343. JBC. 276(29): 27462-27469. 8. Nelson, J.M., & Fry, D.W. (2001) Akt, MAPK (Erk1/2), and p38 act in concert to promote apoptosis in response to ErbB receptor family inhibition. JBC. 276(18): 14842-14847. 9. Genot, E.M., et al. (2000) The T-Cell receptor regulates Akt (Protein Kinase B)via a pathway involving Rac1 and phosphotidylinositide 3-kinase. Mol. Cell. Biol. 20(15): 5469-5478. 10. Datta, S.R., et al. (1997) Akt phosphoylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell. 91(2): 231-241. 11. del Peso, L., et al. (1997) Interleukin-2-induced phosphoylation of BAD through the protein kinase Akt. Science. 278(5338): 687-689. 12. Dudek, H., et al. (1997) Regulation of neuronal survival by the serine-threonine protein kianse Akt. Science. 275(5300): 661-665.
Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format	Purification: Immunogen affinity purified Buffer System: 0.05% Sodium Azide Purity: Immunogen affinity purified State: Aff - Purified
Specificity	Specificity: AKT [Thr308] Species: Human, Mouse
Gene ID	207
FocusOn and Reviews	Reviews: Antibody Panel to AKT Protein Family - Product Review 10
Targets	AKT1 / PKB antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for AKT1 / PKB (9 products)

Catalog No.	Species	Pres.	Purity	Source
AR08011PU-N	AKT1 / PKB (Substrate)
	Human	Purified	> 98 %		0.5 mg / €220.00
					Acris Antibodies GmbH
GTX29041	AKT1 / PKB control peptide
					50 µg / €250.00
					GeneTex Inc.
H00000207-P01-10	AKT1 / PKB
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-P01-25	AKT1 / PKB
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00000207-Q01-10	AKT1 / PKB
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-Q01-25	AKT1 / PKB
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00000207-Q02	AKT1 / PKB
	Human	Purified			10 µg / €330.00
					Abnova Taiwan Corp.
H00000207-Q02-10	AKT1 / PKB
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-Q02-25	AKT1 / PKB
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for AKT1 / PKB (1 products)

Catalog No.	Species
NBL1-07441	AKT1 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for AKT1 / PKB (6 products)

Catalog No.	Host	Pres.
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat	AP
			Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat	Biotin
			Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat	FITC
			Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat	HRP
			Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat	TRITC
			Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey	PE
			Acris Antibodies GmbH