AKT1 / PKB pSer473 - R1465 | acris-antibodies.com

Product Description for AKT1 / PKB

Rabbit anti Chicken, Human, Mouse, Rat AKT1 / PKB.
Properties: pSer473
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for AKT1 / PKB

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, Review 010
Presentation	Aff - Purified
Reactivity	Chk, Hu, Ms, Rt
Applications	E, ICC/IF, P, WB
Clonality	Polyclonal
Host	Rabbit
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P31749 Immunogen: A synthetic peptide corresponding to the C-terminus aa 460-480 of human, mouse, rat and chicken AKT proteins conjugated to KLH AA Sequence: `C-H-F-P-Q-F-pS-Y-S-A-S` Genename: 207 Remarks: Human AKT1 protein, 480 aa, has a predicted MW of 55.7 kDa.
Property	pSer473
Antibody type	Ab
Add. information	Protein Sequence: Human AKT1 protein, 480 aa, predicted MW 55.7kDa 1 msdvaivkeg wlhkrgeyik twrpryfllk ndgtfigyke rpqdvdqrea plnnfsvaqc 61 qlmkterprp ntfiirclqw ttviertfhv etpeereewt taiqtvadgl kkqeeeemdf 121 rsgspsdnsg aeemevslak pkhrvtmnef eylkllgkgt fgkvilvkek atgryyamki 181 lkkevivakd evahtltenr vlqnsrhpfl talkysfqth drlcfvmeya nggelffhls 241 rervfsedra rfygaeivsa ldylhseknv vyrdlklenl mldkdghiki tdfglckegi 301 kdgatmktfc gtpeylapev ledndygrav dwwglgvvmy emmcgrlpfy nqdheklfel 361 ilmeeirfpr tlgpeaksll sgllkkdpkq rlgggsedak eimqhrffag ivwqhvyekk 421 lsppfkpqvt setdtryfde eftaqmitit ppdqddsmec vdserrphfp qfsysasgta
Application	Western blotting (1/200-1/1000). Immunohistochemistry (Formalin-Fixed Paraffin-Embedded Sections, 1/100-1/500) (No pre-treatment of sample is required). ELISA (1/15,000-1/60,000). Immunofluorescence.
Background	AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Concentration	0.75 mg/ml (by UV absorbance at 280 nm)
General Readings	Lawlor MA, Alessi DR. PKB/Akt: a key mediator of cell proliferation, survival and insulin responses? J Cell Sci. 2001 Aug;114(Pt 16):2903-10. PubMed PMID: 11686294. Alessi DR. Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture. Biochem Soc Trans. 2001 May;29(Pt 2):1-14. PubMed PMID: 11356119.
Storage	Store vial at -20°C prior to opening. This product is stable for one month at 2-8°C as an undiluted liquid. Aliquot contents and freeze at -20°C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Expiration date is one year from despatch.
Format	Purification: Immunoaffinity Chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Buffer System: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative. State: Liquid (sterile filtered) purified Ig fraction. Aff - Purified
Specificity	Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated AKT. Minimal reactivity occurs against non-phosphorylated AKT. Species: Human.
Gene ID	207
FocusOn and Reviews	Reviews: Antibody Panel to AKT Protein Family - Product Review 10
Targets	AKT1 / PKB antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for AKT1 / PKB (9 products)

Catalog No.	Species	Pres.	Purity	Source
AR08011PU-N	AKT1 / PKB (Substrate)
	Human	Purified	> 98 %		0.5 mg / €220.00
					Acris Antibodies GmbH
GTX29041	AKT1 / PKB control peptide
					50 µg / €250.00
					GeneTex Inc.
H00000207-P01-10	AKT1 / PKB
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-P01-25	AKT1 / PKB
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00000207-Q01-10	AKT1 / PKB
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-Q01-25	AKT1 / PKB
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00000207-Q02	AKT1 / PKB
	Human	Purified			10 µg / €330.00
					Abnova Taiwan Corp.
H00000207-Q02-10	AKT1 / PKB
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-Q02-25	AKT1 / PKB
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for AKT1 / PKB (1 products)

Catalog No.	Species
NBL1-07441	AKT1 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for AKT1 / PKB (6 products)

Catalog No.	Host	Pres.
R1364AP	Rabbit IgG (H+L chain) antibody
	Goat	AP
			Acris Antibodies GmbH
R1364B	Rabbit IgG (H+L chain) antibody
	Goat	Biotin
			Acris Antibodies GmbH
R1364F	Rabbit IgG (H+L chain) antibody
	Goat	FITC
			Acris Antibodies GmbH
R1364HRP	Rabbit IgG (H+L chain) antibody
	Goat	HRP
			Acris Antibodies GmbH
R1364T	Rabbit IgG (H+L chain) antibody
	Goat	TRITC
			Acris Antibodies GmbH
R1427R	Rabbit IgG F(ab)2 Fragment (H+L chain) antibody
	Donkey	PE
			Acris Antibodies GmbH