AKT1 / PKB pSer473 - SM7001P | acris-antibodies.com

Product Description for AKT1 / PKB

Mouse anti Chicken, Frog, Human, Mouse AKT1 / PKB 104A282.
Properties: pSer473
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for AKT1 / PKB

Product Category	Antibodies
Quantity	0.1 mg
Synonyms	Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase, Review 010
Presentation	Purified
Reactivity	Chk, Frog, Hu, Ms
Applications	WB
Clonality	Monoclonal
Clone	104A282
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P31749 Immunogen: Synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1 Genename: 207
Property	pSer473
Antibody type	Ab
Application	Western blot: 2µg/ml. Immunoprecipitation: 1µg/ml. Recommended Positive Control : PDGF treated NIH 3T3.
Background	Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. Akt/PKB phosphorylation is critical for it activity. The major phosphorylation sites required for Akt activation has been identified as threoinine 308 and serine 473. Serine 473 is phosphorylated by MAPKAP kinase 2.
Concentration	0.5 ml/ml
General Readings	Alessi DR., et al. EMBO J. 15: 6541-6551 (1996). Ferring P., et al. Oncogene 18: 6129-6134 (1999). Kandel ES, et al. Exp. Cell Res. 253: 210-219 (1999). Cantley LC, et al. Proc Natl Acad Sci, USA, 96: 420-4245 (1999). Franke TF, et al. Cell 88: 435-437 (1997).
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: 6 month from despatch.
Format	Purification: Protein G Chromatography Buffer System: PBS containing 0.2% gelatin, 0.05% sodium azide State: Liquid purified IgG Purified
Specificity	Specificity: This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1 Accession no. ref\|NP_005154.2\| (see: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Protein&list_uids=62241011&dopt=GenPept). Clone 104A282 detects specifically phosphorylated form of Akt1 expression in PDGF treated cells. Species: Human, Mouse.
Gene ID	207
FocusOn and Reviews	Reviews: Antibody Panel to AKT Protein Family - Product Review 10
Targets	AKT1 / PKB antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for AKT1 / PKB (9 products)

Catalog No.	Species	Pres.	Purity	Source
AR08011PU-N	AKT1 / PKB (Substrate)
	Human	Purified	> 98 %		0.5 mg / €220.00
					Acris Antibodies GmbH
GTX29041	AKT1 / PKB control peptide
					50 µg / €250.00
					GeneTex Inc.
H00000207-P01-10	AKT1 / PKB
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-P01-25	AKT1 / PKB
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00000207-Q01-10	AKT1 / PKB
	Human			in vitro transl.	10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-Q01-25	AKT1 / PKB
	Human			in vitro transl.	25 µg / €500.00
					Abnova Taiwan Corp.
H00000207-Q02	AKT1 / PKB
	Human	Purified			10 µg / €330.00
					Abnova Taiwan Corp.
H00000207-Q02-10	AKT1 / PKB
	Human	Purified			10 µg / €350.00
					Abnova Taiwan Corp.
H00000207-Q02-25	AKT1 / PKB
	Human	Purified			25 µg / €500.00
					Abnova Taiwan Corp.

Positive controls for AKT1 / PKB (1 products)

Catalog No.	Species
NBL1-07441	AKT1 Lysate
		0.1 mg / €370.00
		Novus Biologicals Inc.

Recommended Secondary Antibodies for AKT1 / PKB (8 products)

Catalog No.	Host	Pres.
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP
			Acris Antibodies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin
			Acris Antibodies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC
			Acris Antibodies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP
			Acris Antibodies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red
			Acris Antibodies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE
			Acris Antibodies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
			Acris Antibodies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
			Acris Antibodies GmbH