AP03032PU-N AKT2 / PKB beta specific antibody

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0.4 ml / €340.00
Delivery Time: 5-7 working days

Quick Overview

Rabbit anti Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus AKT2 / PKB beta

Product Description for AKT2 / PKB beta

Rabbit anti Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Xenopus AKT2 / PKB beta.
Properties: specific
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for AKT2 / PKB beta

Product Category Antibodies
Quantity 0.4 ml
Synonyms Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase, Review 010
Presentation Purified
Reactivity Bov, Can, Chk, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P31751
Immunogen:
A five residue synthetic peptide based on the Human Akt2, coupled to KLH
Genename:
208
Property specific
Antibody type Ab
Application ELISA. 
Western blot: 1/13000 dilution of AP03032PU-N was ufficient for detection of PKB-beta in 20 μg of HeLa cell lysate by ECL immunoblot analysis.
Background The serine/threonine kinase Akt (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "Akt family": Akt1, Akt2, and Akt3.
AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism.
Concentration 0.25 mg/ml
General Readings
  • Downward J (1998). Curr Opin Cell Biol. 10(2): 262-7.
  • Brazil DP, and Hemmings BA (2001). Biochem Sci. 26(11):657-64.
  • Nicholson KM, Anderson NG (2002). Cell Signal. 14(5): 381-95.
  • Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Affinity Chromatography
Buffer System:
TBS
Preservatives:
0.09% Sodium Azide
Stabilizers:
50% Glycerol
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
This antibody detects a ~65kDa protein, corresponding to the molecular mass of Akt2 PKB beta on SDSPAGE immunoblots. Does not react with PKB-alpha (Akt1) or PKB-gamma (Akt3).
Species:
Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus.
Gene ID 208
FocusOn and Reviews
Reviews:
Targets

Accessory Products

Proteins and/or Positive Controls

Proteins for AKT2 / PKB beta (2 products)

Catalog No. Species Pres. Purity   Source  
H00000208-Q01-10

AKT2 / PKB beta

AKT2 / PKB beta Human in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00000208-Q01-25

AKT2 / PKB beta

AKT2 / PKB beta Human in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for AKT2 / PKB beta (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-07442

AKT2 Lysate

Western Blot: AKT2 Lysate [NBL1-07442] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for AKT2
0.1 mg / €500.00
  Novus Biologicals Inc.