Product Description for AKT2 / PKB beta
Rabbit anti Human, Mouse, Rat AKT2 / PKB beta.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.
Properties for AKT2 / PKB beta
| Product Category | Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, RAC-beta serine/threonine-protein kinase, Review 010 |
| Presentation | Aff - Purified |
| Reactivity | Hu, Ms, Rt |
| Applications | ICC/IF, P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Antibody type | Ab |
| Add. information | Research Areas: Cancer |
| Application | immunofluorescence, immunohistochemistry, Western Blot, This antibody is useful for Western Blot, Immunohistochemistry and Immunofluorescence. Western blot analyzes of Akt2 (T468) antibody in extracts from K562 cells. Immunohistochemistry analyzes of Akt2 (T468) antibody in paraffin-embedded human lung carcinoma tissue., Western Blot 1:500-1:1000, immunohistochemistry 1:50-1:200, immunofluorescence 1:50-1:200, |
| Background | The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBbeta or RacPK-beta) and Akt3 (also designated PKBgamma or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-beta tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-I (IGF-I) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-I-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor Wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases. Akt3 is phosphorylated on a serine residue in response to insulin, and this activation is inhibited by prior activation of protein kinase C. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Format |
|
| Specificity |
|
| Gene ID | 208 |
| FocusOn and Reviews | |
| Targets |
Accessory Products
Proteins and/or Positive Controls
Proteins for AKT2 / PKB beta (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| H00000208-Q01-10 |
AKT2 / PKB beta |
||||||
 |
Human | in vitro transl. |
10 µg /
€350.00
|
||||
| Abnova Taiwan Corp. | |||||||
| H00000208-Q01-25 |
AKT2 / PKB beta |
||||||
|
Human | in vitro transl. |
25 µg /
€500.00
|
||||
| Abnova Taiwan Corp. | |||||||
Positive controls for AKT2 / PKB beta (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-07442 |
AKT2 Lysate |
||||||
![Western Blot: AKT2 Lysate [NBL1-07442] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for AKT2](http://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/AKT2 Lysate-Western Blot-NBL1-07442-img0001.jpg) |
0.1 mg /
€500.00
|
||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for AKT2 / PKB beta (6 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1364AP |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | AP | |||||
| Acris Antibodies GmbH | |||||||
| R1364B |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | Biotin | |||||
| Acris Antibodies GmbH | |||||||
| R1364F |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | FITC | |||||
| Acris Antibodies GmbH | |||||||
| R1364HRP |
Rabbit IgG (H+L chain) antibody |
||||||
|
Goat | HRP | |||||
| Acris Antibodies GmbH | |||||||
| R1364T |
Rabbit IgG (H+L chain) antibody |
||||||
|
|
Goat | TRITC | |||||
| Acris Antibodies GmbH | |||||||
| R1427R |
Rabbit IgG F(ab)2 Fragment (H+L chain) antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
Compare Products
You have no items to compare.
My Cart
You have no items in your shopping cart.
Antibody Location Service
Over 230,000 products but you can't find the right antibody for your protein or application?
Acris will do the search for you!
