AP20048PU-N ATG12 / APG12 (Center) antibody

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0.1 mg / €290.00
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Rabbit anti Human, Mouse, Rat ATG12 / APG12

AP20048PU-N

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  • AP20048PU-N

Product Description for ATG12 / APG12

Rabbit anti Human, Mouse, Rat ATG12 / APG12.
Properties: (Center)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for ATG12 / APG12

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O94817
Immunogen:
A 15 amino acid peptide from near the center of Human ATG12
Genename:
9140
Property Center
Application ELISA.
Western blot: ATG12 antibody can be used for the detection of ATG10 at 0.5–1 μg/ml.
Immunohistochemistry on paraffin sections.
Positive Control. Mouse Heart Tissue Lysate.
Background Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
General Readings
  1. Gozuacik D, Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004 Apr 12;23(16):2891-906. PubMed PMID: 15077152.
  2. Kisen GO, Tessitore L, Costelli P, Gordon PB, Schwarze PE, Baccino FM, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis. 1993 Dec;14(12):2501-5. PubMed PMID: 8269618.
  3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13. 4. Hanada T, Noda NN, Satomi Y, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J. Biol. Chem. 2007; 282:37298-302.
  4. Jounai N, Takeshita F, Kobiyama K, Sawano A, Miyawaki A, Xin KQ, et al. The Atg5 Atg12 conjugate associates with innate antiviral immune responses. Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):14050-5. Epub 2007 Aug 20. PubMed PMID: 17709747. (Free PMC Article available, 5 images available)
  5. Nemoto T, Tanida I, Tanida-Miyake E, Minematsu-Ikeguchi N, Yokota M, Ohsumi M, et al. The mouse APG10 homologue, an E2-like enzyme for Apg12p conjugation, facilitates MAP-LC3 modification. J Biol Chem. 2003 Oct 10;278(41):39517-26. Epub 2003 Jul 30. PubMed PMID: 12890687.
Storage Store the antibody undiluted at 2-8°C.
Antibodies should not be exposed to prolonged high temperatures.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography purified via peptide column
Buffer System:
PBS containing 0.02% Sodium Azide as preservative
State:
Liquid purified IgG fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse and Rat
Specificity
Specificity:
Recognizes APG12/ATG12.
Gene ID 9140
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for ATG12 / APG12 (6 products)

Catalog No. Species Pres. Purity   Source  
GTX48095-PRO

ATG12 / APG12

ATG12 / APG12 E. coli
50 µg / €360.00
  GeneTex Inc.
H00009140-P01-10

ATG12 / APG12

ATG12 / APG12 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €350.00
  Abnova Taiwan Corp.
H00009140-P01-25

ATG12 / APG12

ATG12 / APG12 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €500.00
  Abnova Taiwan Corp.
H00009140-P02

ATG12 / APG12

ATG12 / APG12 Human Purified
10 µg / €420.00
  Abnova Taiwan Corp.
H00009140-P02-10

ATG12 / APG12

ATG12 / APG12 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €350.00
  Abnova Taiwan Corp.
H00009140-P02-25

ATG12 / APG12

ATG12 / APG12 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €500.00
  Abnova Taiwan Corp.

Positive controls for ATG12 / APG12 (1 products)

Catalog No. Species Pres. Purity   Source  
NBL1-07795

ATG12 Lysate

Western Blot: ATG12 Lysate [NBL1-07795] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for ATG12
0.1 mg / €370.00
  Novus Biologicals Inc.
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