Apolipoprotein B (Apo B) - BP2056HRP | acris-antibodies.com

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Product Description for Apolipoprotein B (Apo B)

Goat anti Human Apolipoprotein B (Apo B).
Presentation: HRP
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Apolipoprotein B (Apo B)

Product Category	Antibodies
Quantity	1 ml
Synonyms	Apo-B, ApoB, ApoB-100, ApoB100, FocusOn036
Presentation	HRP
Reactivity	Hu
Applications	E, WB
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	Acris Antibodies GmbH

Datasheet Extract

Immunogen	Swiss Prot Num: P04114 Immunogen: Human LDL. Genename: 338
Antibody type	Ab
Application	Can be used to detect the existence of Apo B-48/100 in plasma and lipoproteins for Immunoassay and Immunoblot. Dilution range for Immunoblot and ELISA: 1/2,500-1/10,000.
Background	Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/l.
Caution	Use of Sodium Azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Concentration	1.0 mg/ml (prior to lyophilization)
General Readings	Ng CJ, Bourquard N, Grijalva V, Hama S, Shih DM, Navab M, et al. Paraoxonase-2 deficiency aggravates atherosclerosis in mice despite lower apolipoprotein-B-containing lipoproteins: anti-atherogenic role for paraoxonase-2. J Biol Chem. 2006 Oct 6;281(40):29491-500. Epub 2006 Aug 4. PubMed PMID: 16891303. Herscovitz H, Derksen A, Walsh MT, McKnight CJ, Gantz DL, Hadzopoulou-Cladaras M, et al. The N-terminal 17% of apoB binds tightly and irreversibly to emulsions modeling nascent very low density lipoproteins. J Lipid Res. 2001 Jan;42(1):51-9. PubMed PMID: 11160365. Kitchens RL, Thompson PA, Munford RS, O'Keefe GE. Acute inflammation and infection maintain circulating phospholipid levels and enhance lipopolysaccharide binding to plasma lipoproteins. J Lipid Res. 2003 Dec;44(12):2339-48. Epub 2003 Aug 16. PubMed PMID: 12923224. Lin MC, Gordon D, Wetterau JR. Microsomal triglyceride transfer protein (MTP) regulation in HepG2 cells: insulin negatively regulates MTP gene expression. J Lipid Res. 1995 May;36(5):1073-81. PubMed PMID: 7658155. Reardon CA, Miller ER, Blachowicz L, Lukens J, Binder CJ, Witztum JL, et al. Autoantibodies to OxLDL fail to alter the clearance of injected OxLDL in apolipoprotein E-deficient mice. J Lipid Res. 2004 Jul;45(7):1347-54. Epub 2004 Apr 21. PubMed PMID: 15102879. Schneider, M., et al., (2005), ''High-level lipoprotein(a) expression in transgenic mice: evidence for oxidized phospholipids in lipoprotein(a) but not in low density lipoproteins'', Journal of Lipid Research, 46: 769-778
Storage	Store the lyophilized antibody at 2-8°C. Store the antibody after reconstitution for several weeks at 2-8°C. Prepare working dilution only prior to immediate use. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20°C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Label: Horseradish Peroxidase HRP Purification: Human Apolipoprotein B-100 Sepharose Affinity Chromatography. Buffer System: 50 mM PBS, 0.1M Sodium Chloride, pH 7.4 containing 10 mg/ml BSA as stabilizer and 0.01% Thimerosal as preservative. State: Lyophilized purified Ig fraction. Reconstitution: Restore with 1.0 ml distilled water. Centrifuge product if not completely clear after standing for 1-2 hours at room temperature.
Specificity	Specificity: Binds to Human Apo B-48/100. No cross- reaction with human Apo AI, Apo AII, Apo CI, Apo CII, Apo CIII and Apo E.
Gene ID	338
FocusOn and Reviews	FocusOns: Antibodies to Apolipoprotein - FocusOn 036
Targets	Apolipoprotein B (Apo B) antibody

Accessory Products

Proteins and/or Positive Controls

Proteins for Apolipoprotein B (Apo B) (5 products)

Catalog No.	Species	Pres.	Purity		Source
BA054	Apolipoprotein B (Apo B)
	Human	Purified	> 95 %		Plasma		0.5 mg / €220.00
							Acris Antibodies GmbH
BA1030	Apolipoprotein B (Apo B) (APOB 100)
	Human	Purified			Plasma		1 mg / €250.00
							Acris Antibodies GmbH
H00000338-Q01-10	Apolipoprotein B (Apo B)
	Human	Purified					10 µg / €350.00
							Abnova Taiwan Corp.
H00000338-Q01-25	Apolipoprotein B (Apo B)
	Human	Purified					25 µg / €500.00
							Abnova Taiwan Corp.
PY-20102	Apolipoprotein B (Apo B) (ApoB 100)
	Human	Purified	> 80 %		E. coli		1 mg / €550.00
							Genesis Biotech Inc.

Recommended Secondary Antibodies for Apolipoprotein B (Apo B) (6 products)

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R1317AP	Goat IgG (H+L chain) antibody
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R1317F	Goat IgG (H+L chain) antibody
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R1317HRP	Goat IgG (H+L chain) antibody
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R1317T	Goat IgG (H+L chain) antibody
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R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey		PE
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